Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition.
Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition.
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DOI：
10.1083/jcb.200705160
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发表时间：
2007-12-31
期刊：
影响因子：
--
通讯作者：
Bi E
中科院分区：
文献类型：
--
作者：
Tong Z;Gao XD;Howell AS;Bose I;Lew DJ;Bi E
芽殖酵母酿酒酵母（Saccharomyces cerevisiae）的细胞出生时携带局部化的跨膜标志蛋白，其指导随后建立极性轴，并因此极化生长以在下一个细胞周期中形成芽。在单倍体细胞中，相关的标志性蛋白质集中在前一次细胞分裂的位点，它们在那里招募Cdc24，保守的极性调节剂Cdc42的鸟嘌呤核苷酸交换因子。然而，代替在分裂位点处极化，新的极性轴被引导到该位点旁边但不与该位点重叠。在这里，我们表明，Cdc42鸟苷三磷酸酶激活蛋白（GAP）Rga1建立了一个排除区的分裂网站，阻止随后的极化该网站内。在没有局部Rga1 GAP活性的情况下，新的芽实际上在旧的分裂位点内形成。因此，Cdc42激活剂和GAP建立了同心的作用区，使得极化被引导到邻近但不在先前的细胞分裂位点内发生。
Cells of the budding yeast Saccharomyces cerevisiae are born carrying localized transmembrane landmark proteins that guide the subsequent establishment of a polarity axis and hence polarized growth to form a bud in the next cell cycle. In haploid cells, the relevant landmark proteins are concentrated at the site of the preceding cell division, to which they recruit Cdc24, the guanine nucleotide exchange factor for the conserved polarity regulator Cdc42. However, instead of polarizing at the division site, the new polarity axis is directed next to but not overlapping that site. Here, we show that the Cdc42 guanosine triphosphatase–activating protein (GAP) Rga1 establishes an exclusion zone at the division site that blocks subsequent polarization within that site. In the absence of localized Rga1 GAP activity, new buds do in fact form within the old division site. Thus, Cdc42 activators and GAPs establish concentric zones of action such that polarization is directed to occur adjacent to but not within the previous cell division site.
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DOI：
10.1083/jcb.200109062
发表时间：
2002-01-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gladfelter AS;Bose I;Zyla TR;Bardes ES;Lew DJ
通讯作者：
Lew DJ
影响因子：
64.8
作者：
MORTIMER, RK;JOHNSTON, JR
通讯作者：
JOHNSTON, JR
影响因子：
64.8
作者：
Rittinger, K;Walker, PA;Smerdon, SJ
通讯作者：
Smerdon, SJ
影响因子：
9.2
作者：
Korinek, WS;Bi, E;Chant, J
通讯作者：
Chant, J
影响因子：
3.7
作者：
JOHNSTON, GC;PRINGLE, JR;HARTWELL, LH
通讯作者：
HARTWELL, LH