Cloning rabbits from fetal fibroblasts
Cloning rabbits from fetal fibroblasts
复制标题
从胎儿成纤维细胞克隆兔
DOI：
10.1016/j.livsci.2008.07.019
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发表时间：
2009-05
影响因子：
1.8
通讯作者：
Du, Lixin
中科院分区：
文献类型：
--
作者：
Sheng, Hui Z.;Li, Shangang;Chen, Xuejin;Shi, Jianjun;Guo, Yi;Yin, Chunguang;Du, Lixin
用成年细胞作为核供体，通过核移植产生活兔。我们发现，除了成体细胞核外，培养的胎兔成纤维细胞在核移植到去核卵母细胞后可以支持完整的胚胎发育。核移植后，24.4%(21/86)的胚胎发育到囊胚期，289个胚胎移植到11个受体母亲的输卵管，共6个妊娠。三名母亲怀孕至足月，三只新生的兔子随后通过剖腹产出生，其中一只存活了4个多月。DNA分析证实，所有3只兔子在基因上都与胎儿供体细胞相同。这项研究表明，可以从胎儿成纤维细胞克隆出兔子，尽管克隆的频率低于使用成年体细胞作为核供体时的频率。
Live rabbits were generated through nuclear transfer using adult cells as nuclear donors. We showed that, in addition to adult cell nuclei, cultured fetal rabbit fibroblasts could support full-term embryonic development following nuclear transfer to enucleated oocytes. Following nuclear transfer, 24.4% (21/86) of resulting embryos developed to the blastocyst stage, and 289 embryos were transferred to oviducts of 11 recipient mothers, resulting in 6 pregnancies. Three mothers carried the pregnancy to term, and three newborn rabbits were subsequently delivered by caesarian, one of which survived for more than 4 months. DNA analyses confirmed that all 3 rabbits were genetically identical to fetal donor cells. This study demonstrated that rabbits could be cloned from fetal fibroblasts, although at a lower frequency than when using adult somatic cells as nuclear donors.
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影响因子：
3.6
作者：
Dinnyés, A;Dal, YP;Yang, XZ
通讯作者：
Yang, XZ
影响因子：
56.9
作者：
Lai, LX;Kolber-Simonds, D;Prather, RS
通讯作者：
Prather, RS
DOI：
10.1530/jrf.0.0910113
发表时间：
1991-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CARNEY, EW;FOOTE, RH
通讯作者：
FOOTE, RH
影响因子：
3.8
作者：
Yang, Feikun;Hao, Ru;Zakhartchenko, Valeri
通讯作者：
Zakhartchenko, Valeri
影响因子：
3.1
作者：
Kerns, JA;Olivier, M;Barsh, GS
通讯作者：
Barsh, GS