Stacks off tracks: a role for the golgin AtCASP in plant endoplasmic reticulum-Golgi apparatus tethering.
Stacks off tracks: a role for the golgin AtCASP in plant endoplasmic reticulum-Golgi apparatus tethering.
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DOI：
10.1093/jxb/erx167
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发表时间：
2017-06-15
影响因子：
6.9
通讯作者：
Hawes C
中科院分区：
文献类型：
--
作者：
Osterrieder A;Sparkes IA;Botchway SW;Ward A;Ketelaar T;de Ruijter N;Hawes C
高尔基体相关的拟南芥蛋白AtCASP可能形成一个高尔基体介导的拴系复合物的一部分，参与锚定植物高尔基体堆栈的内质网（ER）。植物高尔基体修饰和分类来自内质网（ER）的蛋白质并合成细胞壁基质材料。植物细胞具有许多可动的高尔基体，其通过尚未鉴定的栓系因子连接到ER。以往的研究表明，在高尔基体的生物合成中，顺式高尔基体植物golgins，这是一种长的卷曲螺旋结构域蛋白锚定高尔基体膜。在这里，我们显示了一个拴系作用的高尔基体AtCASP在ER高尔基体接口。使用活细胞成像，在表达荧光标记的AtCASP、缺少卷曲螺旋结构域的截短AtCASP-Δ CC和高尔基体标记STtmd的拟南芥叶表皮细胞中比较高尔基体动力学。AtCASP-Δ CC系中高尔基体的速度和位移显著降低。利用双色光学捕获系统和TIRF镊子系统，在植物中捕获单个高尔基体。AtCASP-Δ CC系中的高尔基体更容易被捕获，内质网-高尔基体连接更容易被破坏。偶尔，内质网小管跟随一个被困的高尔基体与间隙，表明存在其他拴系因子。我们的工作证实，ER-高尔基体的紧密结合可以被截短的AtCASP-Δ CC的表达破坏或减弱，并表明这种连接很可能是由高尔基体介导的束缚复合物维持的。
The Golgi-associated Arabidopsis thaliana protein AtCASP may form part of a golgin-mediated tethering complex involved in anchoring plant Golgi stacks to the endoplasmic reticulum (ER). The plant Golgi apparatus modifies and sorts incoming proteins from the endoplasmic reticulum (ER) and synthesizes cell wall matrix material. Plant cells possess numerous motile Golgi bodies, which are connected to the ER by yet to be identified tethering factors. Previous studies indicated a role for cis-Golgi plant golgins, which are long coiled-coil domain proteins anchored to Golgi membranes, in Golgi biogenesis. Here we show a tethering role for the golgin AtCASP at the ER-Golgi interface. Using live-cell imaging, Golgi body dynamics were compared in Arabidopsis thaliana leaf epidermal cells expressing fluorescently tagged AtCASP, a truncated AtCASP-ΔCC lacking the coiled-coil domains, and the Golgi marker STtmd. Golgi body speed and displacement were significantly reduced in AtCASP-ΔCC lines. Using a dual-colour optical trapping system and a TIRF-tweezer system, individual Golgi bodies were captured in planta. Golgi bodies in AtCASP-ΔCC lines were easier to trap and the ER-Golgi connection was more easily disrupted. Occasionally, the ER tubule followed a trapped Golgi body with a gap, indicating the presence of other tethering factors. Our work confirms that the intimate ER-Golgi association can be disrupted or weakened by expression of truncated AtCASP-ΔCC and suggests that this connection is most likely maintained by a golgin-mediated tethering complex.
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影响因子：
3.3
作者：
Ito Y;Uemura T;Shoda K;Fujimoto M;Ueda T;Nakano A
通讯作者：
Nakano A
影响因子：
4.3
作者：
Gilson, PR;Vergara, CE;Gleeson, PA
通讯作者：
Gleeson, PA
影响因子：
3.3
作者：
Gillingham, AK;Pfeifer, AC;Munro, S
通讯作者：
Munro, S
影响因子：
2.9
作者：
Kang, Byung-Ho;Staehelin, L. Andrew
通讯作者：
Staehelin, L. Andrew
影响因子：
3.9
作者：
Hawes, Chris;Schoberer, Jennifer;Osterrieder, Anne
通讯作者：
Osterrieder, Anne