The zebrafish dyrk1b gene is important for endoderm formation.
The zebrafish dyrk1b gene is important for endoderm formation.
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DOI：
10.1002/dvg.20578
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发表时间：
2010-01
期刊：
影响因子：
1.5
通讯作者：
Nissen, Robert M.
中科院分区：
文献类型：
--
作者：
Mazmanian, Gohar;Kovshilovsky, Michael;Yen, Debbie;Mohanty, Aditya;Mohanty, Sudipta;Nee, Alex;Nissen, Robert M.
节点信号是需要规范的中胚层，内胚层，建立左右不对称和颅面发育。Wdr 68是一种含有WD 40重复结构域的蛋白质，最近被证明是内皮素-1（edn 1）表达和随后的下颌发育所必需的。以前的报告检测到Wdr 68蛋白的多蛋白复合物含有哺乳动物成员的双特异性酪氨酸调节激酶（dyrk）家族。在这里，我们描述的斑马鱼dyrk 1b同源物的特性。我们报告检测Dyrk 1b和Wdr 68之间的物理相互作用。我们还发现了dyrk 1b反义吗啉代敲低（dyrk 1b-MO）动物中Nodal信号的扰动。具体来说，我们发现lft 1和lft 2（lft 1/2）在原肠胚形成过程中的表达减少，并且后来的不对称lft 1/2表达结构域几乎完全丧失。尽管wdr 68-MO动物在原肠胚形成过程中没有显示lft 1/2表达缺陷，但它们显示了后期不对称lft 1/2表达结构域的几乎完全丧失。虽然ndr 1的表达在原肠胚形成过程中没有受到实质性影响，但在dyrk 1b-MO动物中ndr 2的表达中度降低。对dyrk 1b-MO动物中Nodal信号通路的其他下游组分的分析显示，背轴中胚层标记物gsc的表达适度扩大，而泛中胚层标记物bik基本上不受影响。dyrk 1b-MO动物的内胚层标志物cas和sox 17也中度降低。值得注意的是，与先前报道的wdr 68突变动物的缺陷相似，我们还发现dyrk 1b-MO动物中咽囊标记edn 1的表达减少。总之，这些数据揭示了dyrk 1b在斑马鱼内胚层形成和颅面图案中的作用。
Nodal-signaling is required for specification of mesoderm, endoderm, establishing left-right asymmetry and craniofacial development. Wdr68 is a WD40-repeat domain-containing protein recently shown to be required for endothelin-1 (edn1) expression and subsequent lower jaw development. Previous reports detected the Wdr68 protein in multiprotein complexes containing mammalian members of the dual-specificity tyrosine-regulated kinase (dyrk) family. Here we describe the characterization of the zebrafish dyrk1b homolog. We report the detection of a physical interaction between Dyrk1b and Wdr68. We also found perturbations of Nodal signaling in dyrk1b antisense morpholino knockdown (dyrk1b-MO) animals. Specifically, we found reduced expression of lft1 and lft2 (lft1/2) during gastrulation and a near complete loss of the later asymmetric lft1/2 expression domains. Although wdr68-MO animals did not display lft1/2 expression defects during gastrulation, they displayed a near complete loss of the later asymmetric lft1/2 expression domains. While expression of ndr1 was not substantially effected during gastrulation, ndr2 expression was moderately reduced in dyrk1b-MO animals. Analysis of additional downstream components of the Nodal signaling pathway in dyrk1b-MO animals revealed modestly expanded expression of the dorsal axial mesoderm marker gsc while the pan-mesodermal marker bik was largely unaffected. The endodermal markers cas and sox17 were also moderately reduced in dyrk1b-MO animals. Notably, and similar to defects previously reported for wdr68 mutant animals, we also found reduced expression of the pharyngeal pouch marker edn1 in dyrk1b-MO animals. Taken together, these data reveal a role for dyrk1b in endoderm formation and craniofacial patterning in the zebrafish.
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影响因子：
9.8
作者：
Crump JG;Swartz ME;Kimmel CB
通讯作者：
Kimmel CB
影响因子：
4.8
作者：
Jin, Kideok;Ewton, Daina Z.;Friedman, Eileen
通讯作者：
Friedman, Eileen
DOI：
10.1006/bbrc.1998.9967
发表时间：
1999-01-19
影响因子：
3.1
作者：
Leder, S;Weber, Y;Becker, W
通讯作者：
Becker, W
影响因子：
2.7
作者：
Clouthier, DE;Williams, SC;Yanagisawa, M
通讯作者：
Yanagisawa, M
影响因子：
4.6
作者：
Long, S;Ahmad, N;Rebagliati, M
通讯作者：
Rebagliati, M