An integrin-dependent role of pouch endoderm in hyoid cartilage development.
An integrin-dependent role of pouch endoderm in hyoid cartilage development.
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DOI：
10.1371/journal.pbio.0020244
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发表时间：
2004-09
期刊：
影响因子：
9.8
通讯作者：
Kimmel CB
中科院分区：
文献类型：
--
作者：
Crump JG;Swartz ME;Kimmel CB
咽内胚层对于头部骨骼的发育是必不可少的，并且可以重新编程。在这里，我们调查特定的内胚层结构在调节颅面发育中的作用。我们已经在斑马鱼中分离出一种整合素α5突变体，该突变体具有来自舌骨神经嵴细胞的面部软骨的区域特异性损失。此外，在整合素α5−动物中，通常附着于受影响软骨区域的颅肌及其相关神经继发性减少。在发育早期，整合素α5突变体在第一个囊袋（咽内胚层的外囊）的形成中也有特定的缺陷。通过命运定位，我们发现整合素α5突变体中丢失的软骨区域是从野生型动物中直接邻近第一个囊袋的神经嵴细胞发育而来的。此外，我们证明了整合素α5在内胚层中起控制囊形成和软骨发育的作用。时间推移记录表明，第一袋促进区域特定的软骨发育，通过调节局部压实和生存的成骨神经嵴细胞。因此，我们的研究结果揭示了组织相互作用的层次结构，在其顶部是第一个内胚层袋，其局部协调脊椎动物面部特定区域中多个组织的发育。最后，我们讨论了马赛克装配的面部骨骼的进化的鳍鱼的影响。斑马鱼的一种发育突变体正在帮助揭示脊椎动物面部骨骼的形成过程
Pharyngeal endoderm is essential for and can reprogram development of the head skeleton. Here we investigate the roles of specific endodermal structures in regulating craniofacial development. We have isolated an integrinα5 mutant in zebrafish that has region-specific losses of facial cartilages derived from hyoid neural crest cells. In addition, the cranial muscles that normally attach to the affected cartilage region and their associated nerve are secondarily reduced in integrinα5− animals. Earlier in development, integrinα5 mutants also have specific defects in the formation of the first pouch, an outpocketing of the pharyngeal endoderm. By fate mapping, we show that the cartilage regions that are lost in integrinα5 mutants develop from neural crest cells directly adjacent to the first pouch in wild-type animals. Furthermore, we demonstrate that Integrinα5 functions in the endoderm to control pouch formation and cartilage development. Time-lapse recordings suggest that the first pouch promotes region-specific cartilage development by regulating the local compaction and survival of skeletogenic neural crest cells. Thus, our results reveal a hierarchy of tissue interactions, at the top of which is the first endodermal pouch, which locally coordinates the development of multiple tissues in a specific region of the vertebrate face. Lastly, we discuss the implications of a mosaic assembly of the facial skeleton for the evolution of ray-finned fish. A developmental mutant of the zebrafish is helping to uncover the processes by which the facial skeleton is patterned in vertebrates
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影响因子：
64.8
作者：
HUNT, P;GULISANO, M;KRUMLAUF, R
通讯作者：
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