Hepatocyte growth factor elevates the activity levels of glycolipid sulfotransferases in renal cell carcinoma cells.
Hepatocyte growth factor elevates the activity levels of glycolipid sulfotransferases in renal cell carcinoma cells.
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肝细胞生长因子可提高肾细胞癌细胞中糖脂磺基转移酶的活性水平。
DOI：
--
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Akira Makita
中科院分区：
文献类型：
--
作者：
Takahiko Kobayashi;Koichi Honke;Shinsei Gasa;T. Miyazaki;H. Tajima;Kunio Matsumoto;Toshikazu Nakamura;Akira Makita
先前已在人肾细胞癌细胞系 SMKT-R3 中证实，磺基糖脂的积累与糖脂磺基转移酶的活性水平显着升高相关。为了阐明SMKT-R3细胞中磺基糖脂合成的调节机制，研究了各种生长因子对磺基糖脂代谢酶的影响。肝细胞生长因子 (HGF) 以剂量依赖性方式显着增加磺基转移酶的活性水平，但不会改变水解磺基糖脂的芳基硫酸酯酶 A 的活性水平。 Scatchard 对 125I-HGF 与 SMKT-R3 细胞结合的分析表明，这些细胞表达 HGF 的高亲和力受体，Kd 为 36 pM，每个细胞有 750 个位点。此外，[35S]硫酸盐代谢标记表明，向细胞培养基中添加HGF导致磺基糖脂合成增加。因此，这些观察结果表明，HGF 可以通过调节肾细胞癌细胞中的磺基转移酶活性水平，充当磺基糖脂合成的调节因子。此外，HGF刺激SMKT-R3细胞的增殖和运动，表明HGF在肾细胞癌细胞中具有多种生物学活性。
Accumulation of sulfoglycolipids associated with markedly elevated activity levels of glycolipid sulfotransferases has previously been demonstrated in the human renal cell carcinoma cell line, SMKT-R3. To elucidate the regulatory mechanisms of sulfoglycolipid synthesis in SMKT-R3 cells, the effects of various growth factors on the metabolic enzymes of sulfoglycolipids were investigated. Hepatocyte growth factor (HGF) significantly increased the activity levels of the sulfotransferases in a dose-dependent manner, but did not change that of arylsulfatase A, which hydrolyzes sulfoglycolipids. Scatchard analysis of 125I-HGF binding to SMKT-R3 cells indicated that the cells expressed high-affinity receptors for HGF with a Kd of 36 pM and 750 sites/cell. Furthermore, metabolic labeling with [35S]sulfate revealed that the addition of HGF to the culture medium of the cells resulted in an increment of sulfoglycolipid synthesis. Therefore, these observations suggest that HGF can function as a regulatory factor in sulfoglycolipid synthesis through the modulation of the sulfotransferase activity levels in renal cell carcinoma cells. In addition, HGF stimulated the proliferation and motility of SMKT-R3 cells, suggesting that HGF has multiple biological activities in renal cell carcinoma cells.
哺乳动物精子发生过程中半乳甘油脂和半乳鞘脂硫酸化的发育调节。
DOI：
10.1042/bj2310393
发表时间：
1985
期刊：
The Biochemical journal
影响因子：
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作者：
Lingwood,CA
通讯作者：
Lingwood,CA
影响因子：
56.9
作者：
AARONSON, SA
通讯作者：
AARONSON, SA