A H2 very high frequency capacitively coupled plasma inactivates glyceraldehyde 3-phosphate dehydrogenase(GapDH) more efficiently than UV photons and heat combined
A H2 very high frequency capacitively coupled plasma inactivates glyceraldehyde 3-phosphate dehydrogenase(GapDH) more efficiently than UV photons and heat combined
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H2 甚高频电容耦合等离子体比紫外光子和热结合更有效地灭活 3-磷酸甘油醛脱氢酶 (GapDH)
DOI：
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
P. Awakowicz
中科院分区：
文献类型：
--
作者：
K. Stapelmann;J. Lackmann;Ines Buerger;J. Bandow;P. Awakowicz
等离子体灭菌是常用灭菌技术的一种有前途的替代方法，因为常规方法受到某些限制，例如与热敏材料不相容或使用有毒试剂。然而，基于等离子体的灭菌机制尚未完全理解。采用低压甚高频电容耦合等离子体研究了氢气放电对蛋白质甘油醛3-磷酸脱氢酶（GapDH）的影响。GapDH是一种糖酵解酶。作为中央代谢的一部分，它发生在从细菌到人类的几乎所有生物体中。用绝对校准的光学发射光谱法研究血浆，以鉴定和定量可能导致酶失活的血浆组分。分别研究了UV光子和热对GapDH失活的贡献，两者似乎都不是主要因素。为了研究GapDH通过氢放电失活的机制，研究样品的蚀刻、诱导氨基酸骨架断裂和化学修饰。虽然既没有观察到蚀刻也没有观察到链断裂，但化学修饰发生在GapDH的不同氨基酸残基处。在VHF-CCP处理后检测到天冬酰胺的脱酰胺以及甲硫氨酸和半胱氨酸氧化。特别地，已知活性中心中半胱氨酸的氧化导致GapDH失活。
Plasma sterilization is a promising alternative to commonly used sterilization techniques, because the conventional methods suffer from certain limitations, e.g. incompatibility with heat-sensitive materials, or use of toxic agents. However, plasma-based sterilization mechanisms are not fully understood yet. A low-pressure very high frequency capacitively coupled plasma is used to investigate the impact of a hydrogen discharge on the protein glyceraldehyde 3-phosphate dehydrogenase (GapDH). GapDH is an enzyme of glycolysis. As a part of the central metabolism, it occurs in nearly all organisms from bacteria to humans. The plasma is investigated with absolutely calibrated optical emission spectroscopy in order to identify and to quantify plasma components that can contribute to enzyme inactivation. The contribution of UV photons and heat to GapDH inactivation is investigated separately, and neither seems to be a major factor. In order to investigate the mechanisms of GapDH inactivation by the hydrogen discharge, samples are investigated for etching, induction of amino acid backbone breaks, and chemical modifications. While neither etching nor strand breaks are observed, chemical modifications occur at different amino acid residues of GapDH. Deamidations of asparagines as well as methionine and cysteine oxidations are detected after VHF-CCP treatment. In particular, oxidation of the cysteine in the active centre is known to lead to GapDH inactivation.
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影响因子：
2.2
作者：
Kerri Mafia;R. Gupta;M. Kirk;L. Wilson;O. P. Srivastava;S. Barnes
通讯作者：
Kerri Mafia;R. Gupta;M. Kirk;L. Wilson;O. P. Srivastava;S. Barnes
影响因子：
5.6
作者：
Zhao H;Brown PH;Magone MT;Schuck P
通讯作者：
Schuck P
血单核细胞中吞噬作用相关的呼吸爆发诱导的甘油醛-3-磷酸脱氢酶的S-硫醇化。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ravichandran,V;Seres,T;Moriguchi,T;Thomas,JA;JohnstonJr,RB
通讯作者：
JohnstonJr,RB
利用低压等离子体灭活不锈钢螺丝上的细菌孢子
影响因子：
4.2
作者：
Stapelmann K;Fiebrandt M;Raguse M;Awakowicz;Reitz G;Moeller R
通讯作者：
Moeller R
影响因子：
3.9
作者：
Lackmann, Jan-Wilm;Schneider, Simon;Bandow, Julia E.
通讯作者：
Bandow, Julia E.