Comparison of MALDI-TOF mass spectrometry and rpoB gene sequencing for the identification of clinical isolates of Aeromonas spp.
Comparison of MALDI-TOF mass spectrometry and rpoB gene sequencing for the identification of clinical isolates of Aeromonas spp.
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DOI：
10.1016/j.heliyon.2022.e11585
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发表时间：
2022-11
期刊：
影响因子：
4
通讯作者：
Yano, Hisakazu
中科院分区：
文献类型：
--
作者：
Kitagawa, Daisuke;Suzuki, Yuki;Abe, Noriyuki;Ui, Koji;Suzuki, Keitaro;Yamashita, Takaya;Sakaguchi, Atsuko;Suzuki, Soma;Masuo, Kazue;Nakano, Akiyo;Nakano, Ryuichi;Sato, Masatoshi;Maeda, Koichi;Nakamura, Fumihiko;Yano, Hisakazu
气单胞菌广泛存在于河流和土壤中，可引起人类轻度胃肠炎、严重败血症和软组织感染。这些感染的治疗需要准确鉴定致病性气单胞菌属。然而，使用传统方法在物种水平上进行鉴定非常具有挑战性。在本研究中，我们旨在比较两种不同的细菌鉴定方法的准确性：(i) 管家基因测序 (rpoB) 与系统发育分析相结合，以及 (ii) 基质辅助激光解吸电离质谱-飞行时间 (MALDI-TOF MS)（MALDI Biotyper 和 VITEK MS），用于区分气单胞菌属。我们使用两种鉴定方法分析了从日本不同医疗机构的患者中回收的 58 株气单胞菌分离株。 rpoB 测序方法是最准确的，可以在物种水平上识别所有气单胞菌分离株。同时，MALDI Biotyper 系统在属水平上正确识别了 53 个（91.4%）分离株，在种水平上正确识别了另外 30 个（51.7%）分离株。 VITEK MS 系统在属水平正确识别了 58 个分离株（100%），在种水平正确识别了另外 34 个分离株（58.6%）。因此，MALDI Biotyper 和 VITEK MS 在属水平上准确鉴定了分离株，但在物种鉴定的准确性方面存在差异。然而，低成本和易于分析使得基于 MALDI-TOF MS 的方法成为需要易于使用的识别方法的临床实验室的有力候选者。基于质谱 (MS) 的方法对气单胞菌进行了评估。鉴别。看家基因 rpoB 测序最准确地鉴定了气单胞菌属物种。 MALDI Biotyper 和 VITEK MS 的准确度因物种而异。数据库扩展将有助于提高基于MS的方法的识别准确性。气单胞菌；细菌鉴定； rpoB； MALDI-TOF MS； MALDI 生物型；维特克女士
Aeromonas spp., widely present in rivers and soil, cause mild gastroenteritis, severe septicemia, and soft tissue infections in humans. Treatment of these infections require accurate identification of pathogenic Aeromonas spp. However, identification at the species level using conventional methods is highly challenging. In this study, we aimed to compare the accuracy of two different approaches developed for bacterial identification: (i) housekeeping gene sequencing (rpoB) in conjunction with phylogenetic analysis and (ii) matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS) (MALDI Biotyper and VITEK MS), for differentiating Aeromonas spp. We analyzed 58 Aeromonas isolates recovered from patients at different medical institutions in Japan using both identification methods. The rpoB sequencing method was the most accurate, identifying all Aeromonas isolates at the species level. Meanwhile, the MALDI Biotyper system correctly identified 53 (91.4%) isolates at the genus level and an additional 30 (51.7%) at the species level. The VITEK MS system correctly identified 58 (100%) isolates at the genus level and an additional 34 (58.6%) at the species level. Thus, MALDI Biotyper and VITEK MS accurately identified isolates at the genus level, but differences were found in the accuracy of identification of species. However, the low cost and ease of analysis make MALDI-TOF MS-based methods strong candidates for use in clinical laboratories that require easy-to-use identification methods. Mass spectrometry (MS)-based methods evaluated for Aeromonas spp. identification. Housekeeping gene rpoB sequencing most accurately identified Aeromonas species. MALDI Biotyper and VITEK MS differed in accuracy depending on the species. Database extension will help improve identification accuracy of MS-based methods. Aeromonas; Bacterial identification; rpoB; MALDI-TOF MS; MALDI Biotyper; VITEK MS.
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DOI：
10.1099/ijs.0.03048-0
发表时间：
2004-09-01
影响因子：
2.8
作者：
Soler, L;Yáñez, MA;Martínez-Murcia, AJ
通讯作者：
Martínez-Murcia, AJ
影响因子：
2.4
作者：
Shin HB;Yoon J;Lee Y;Kim MS;Lee K
通讯作者：
Lee K
影响因子：
5.2
作者：
Chen PL;Lamy B;Ko WC
通讯作者：
Ko WC
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
39.2
作者：
HOLMBERG, SD;SCHELL, WL;FARMER, JJ
通讯作者：
FARMER, JJ