Polylysine Stimulation of Ectopic Cartilage Formation
Polylysine Stimulation of Ectopic Cartilage Formation
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多聚赖氨酸刺激异位软骨形成
DOI：
--
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
D. Kreitzer
中科院分区：
文献类型：
--
作者：
R. Tuan;Dina M. Turchi;D. Kreitzer
软骨发育或软骨发生是一个过程，其涉及前软骨形成间充质细胞的浓缩，随后是以软骨特异性细胞外基质组分的生物合成为特征的软骨表型的表达。我们以前已经表明，阳离子生物聚合物，聚赖氨酸（PL），是能够刺激软骨形成胚胎肢芽间充质细胞在体外。在这项研究中，我们已经评估了软骨形成刺激活动的PL在体内使用的实验系统，包括鸡胚肌肉外植体，这是移植到绒毛尿囊膜的鸡胚保持在长期无壳培养。肌肉移植物与PL处理的PL通过多次直接注射PL的398 KO先生。在肌肉移植物的表型变化进行了评估，通过组织学，扫描电子显微镜，和细胞外基质成分的生物合成的分析。显微镜观察显示，在PL处理的移植物中详细阐述了软骨样基质，如阳性阿辛蓝染色和存在丰富的基质材料所示，在形态上与典型软骨相似。PL处理的移植物中的软骨基质生物合成进一步通过其增加的[5S]硫酸盐掺入和II型胶原的表观表达来指示。总之，这些发现表明，PL的行为，刺激，或可能诱导，软骨形成的移植肌肉。据推测，基质分子与PL样，阳离子域可能会在体内发挥作用，以调节软骨形成。
Cartilage development, or chondrogenesis, is a process which involves the condensation of prechondrogenic mesenchymal cells, followed by the expression of the cartilage phenotype characteri zed by the biosynthesis of cartilage-specific, extracellular matrix components. We have previously shown that the cationic biopolymer , polylysine (PL) , is able to stimulate chondrogenesis by embryonic limb bud mesenchymal cells in vitro. In this study, we have evaluated the chondrogenesis -stimulating activity of PL in vivo using an experimental system consisting of chick embryonic muscle explants, which were grafted onto the chorioallantoic membrane of chick embryos maintained in long-term shell -less culture. The muscle grafts were treated with PL by multiple direct injections of PL of 398 KO Mr. The phenotypic changes in the muscle grafts were assessed by histology, scanning electron microscopy, and analysis of the biosynthesis of extracellular matrix components. The microscopic observations revealed that a cartilage-like matrix was elaborated in the PL-treated grafts, as indicated by positive alcian blue staining and the presence of abundant matrix material morphologically similar to that in a typical cartilage. Cartilage matrix biosynthesis in the PL-treated grafts was further indicated by their increased f 5S] sulfate incorporation and the apparent expression of collagen type II. Taken together, these findings indicate that PL acts to stimulate, or perhaps induce, chondrogenesis in the explanted muscle grafts. It is speculated that matrix molecules with PL-like, cationic domains may act in vivo to regulate chondrogenesis.
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影响因子：
2.7
作者：
ANTONIO, JDS;TUAN, RS
通讯作者：
TUAN, RS
鸡胚绒毛尿囊膜的碳酸酐酶和钙转运功能。
影响因子：
5.2
作者：
Tuan,RS
通讯作者：
Tuan,RS
影响因子：
2.7
作者：
J. S. Antonio;B. M. Winston;R. Tuan
通讯作者：
J. S. Antonio;B. M. Winston;R. Tuan
实验诱导的缺钙对鸡胚胎骨骼中胶原蛋白类型发育表达的影响。
影响因子：
2.7
作者：
Tuan,RS;Lynch,MH
通讯作者：
Lynch,MH
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hauschka,PV;Mavrakos,AE;Iafrati,MD;Doleman,SE;Klagsbrun,M
通讯作者：
Klagsbrun,M