Macrophages promote the growth and invasion of endometrial stromal cells by downregulating IL-24 in endometriosis.
Macrophages promote the growth and invasion of endometrial stromal cells by downregulating IL-24 in endometriosis.
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子宫内膜异位症中巨噬细胞通过下调IL-24促进子宫内膜基质细胞的生长和侵袭
DOI：
10.1530/rep-16-0278
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发表时间：
2016-12
期刊：
影响因子：
3.8
通讯作者：
Li Ming-Qing
中科院分区：
文献类型：
--
作者：
Shao Jun;Zhang Bing;Yu Jia-Jun;Wei Chun-Yan;Zhou Wen-Jie;Chang Kai-Kai;Yang Hui-Li;Jin Li-Ping;Zhu Xiao-Yong;Li Ming-Qing
Macrophages play an important role in the origin and development of endometriosis. Estrogen promoted the growth of decidual stromal cells (DSCs) by downregulating the level of interleukin (IL)-24. The aim of this study was to clarify the role and mechanism of IL-24 and its receptors in the regulation of biological functions of endometrial stromal cells (ESCs) during endometriosis. The level of IL-24 and its receptors in endometrium was measured by immunohistochemistry. In vitro analysis was used to measure the level of IL-24 and receptors and the biological behaviors of ESCs. Here, we found that the expression of IL-24 and its receptors (IL-20R1 and IL-20R2) in control endometrium was significantly higher than that in eutopic and ectopic endometrium of women with endometriosis. Recombinant human IL-24 (rhIL-24) significantly inhibited the viability of ESCs in a dosage-dependent manner. Conversely, blocking IL-24 with anti-IL-24 neutralizing antibody promoted ESCs viability. In addition, rhIL-24 could downregulate the invasiveness of ESCs in vitro After co-culture, macrophages markedly reduced the expression of IL-24 and IL-20R1 in ESCs, but not IL-22R1. Moreover, macrophages significantly restricted the inhibitory effect of IL-24 on the viability, invasion, the proliferation relative gene Ki-67, proliferating cell nuclear antigen (PCNA) and cyclooxygenase2 (COX-2), and the stimulatory effect on the tumor metastasis suppressor gene CD82 in ESCs. These results indicate that the abnormally low level of IL-24 in ESCs possibly induced by macrophages may lead to the enhancement of ESCs' proliferation and invasiveness and contribute to the development of endometriosis.
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DOI：
10.1073/pnas.152327199
发表时间：
2002-07-23
影响因子：
11.1
作者：
Sarkar, D;Su, ZZ;Fisher, PB
通讯作者：
Fisher, PB
DOI：
10.1210/jcem.87.7.8682
发表时间：
2002-07
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
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作者：
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通讯作者：
M. Ueda;Y. Yamashita;M. Takehara;Y. Terai;K. Kumagai;K. Ueki;K. Kanda;H. Yamaguchi;Daisuke Akise-Da
影响因子：
6.7
作者：
Witz, CA;Thomas, MR;Schenken, RS
通讯作者：
Schenken, RS
DOI：
--
发表时间：
1927-03
期刊：
The American journal of pathology
影响因子：
--
作者：
J. Sampson
通讯作者：
J. Sampson
DOI：
10.1530/jrf.0.0820707
发表时间：
1988-03
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
G. Dunselman;M. Hendrix;P. Bouckaert;J. Evers
通讯作者：
G. Dunselman;M. Hendrix;P. Bouckaert;J. Evers