IL23R as an indicator of immune infiltration and poor prognosis in intrahepatic cholangiocarcinoma: a bioinformatics analysis.
IL23R as an indicator of immune infiltration and poor prognosis in intrahepatic cholangiocarcinoma: a bioinformatics analysis.
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DOI：
10.21037/tcr-23-455
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发表时间：
2023-10-31
影响因子：
0.9
通讯作者：
中科院分区：
文献类型：
--
作者：
虽然肝内胆管癌（CHOL）的发病率很低，但预后很差。白细胞介素23受体（IL23R）的表达水平与癌症的发生和发展有关。本研究旨在利用生物信息学工具和实验验证来确定IL23R在CHOL中的作用。从Gene Expression Omnibus （GEO）数据库中获取环状RNA （circRNA）、microRNA （miRNA）和信使RNA （mRNA）数据集，使用R软件进行数据分析和可视化。使用基因本体（GO）和京都基因与基因组百科全书（KEGG）进行功能富集分析，并使用基因集富集分析软件进行验证。临床数据来自癌症基因组图谱（TCGA），使用DriverDBv3数据库和基因表达谱互动分析网站进行生存分析。TIMER2.0数据库为我们提供了IL23R免疫细胞浸润分析结果。采用实时定量聚合酶链反应（RT-qPCR）验证IL23R的表达。差异表达（DE） mrna富集于磷酸肌肽3-激酶-丝氨酸/苏氨酸激酶信号通路、免疫相关肿瘤微环境（TME）、氨基酸代谢等。此外，IL23R的表达与免疫浸润相关细胞有关。此外，我们还建立了circRNA-miRNA-IL23R网络和IL23R蛋白-蛋白相互作用网络。最重要的是，IL23R作为预后基因，在CHOL中低表达。鉴定出circRNA-miRNA-IL23R网络，发现IL23R可能是CHOL预后和免疫相关的生物标志物，值得进一步探索。
Although the incidence of intrahepatic cholangiocarcinoma (CHOL) is low, the prognosis is very poor. The expression level of interleukin 23 receptor (IL23R) is linked to the occurrence and development of cancers. This study aimed to identify the role of IL23R in CHOL using bioinformatics tools and experimental validation. Circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) datasets were obtained from the Gene Expression Omnibus (GEO) database, and R software was used for data analysis and visualization. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional enrichment analysis, which was verified with gene set enrichment analysis software. Clinical data were obtained from The Cancer Genome Atlas (TCGA), and survival analyses were performed using the DriverDBv3 database and the Gene Expression Profiling Interactive Analysis website. The TIMER2.0 database provided us for immune cell infiltration analysis results of IL23R. Real-time quantitative polymerase chain reaction (RT-qPCR) was used for IL23R expression verification. Differentially expressed (DE) mRNAs were enriched in phosphoinositide 3-kinase-serine/threonine kinase signaling pathway, immune-related tumor microenvironment (TME), and amino acid metabolism, etc. In addition, expression of IL23R was associated with immune infiltration-related cells. Furthermore, a circRNA-miRNA-IL23R network and a IL23R protein-protein interaction network were established. Most importantly, IL23R, as a prognostic gene, was found to have a low expression in CHOL. A circRNA-miRNA-IL23R network was identified, and it was found that IL23R may be a prognostic and immune-related biomarker in CHOL, which is worthy of further exploration.
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影响因子：
5.8
作者：
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