Sensitive detection of dopamine with ultrasound cavitation-enhanced fluorescence method
Sensitive detection of dopamine with ultrasound cavitation-enhanced fluorescence method
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超声空化增强荧光法灵敏检测多巴胺
DOI：
10.1016/j.microc.2019.104199
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发表时间：
2019-11
影响因子：
4.8
通讯作者：
Wu Daocheng
中科院分区：
文献类型：
--
作者：
Zhu Hongrui;Gan Zhenhai;Li Dapeng;Qin Jing;Zhang Hongmei;Wan Mingxi;Wu Daocheng
多巴胺（DA）的检测对于医学诊断至关重要，并且大多数现有方法需要复杂的仪器和长的处理时间。虽然荧光分析方法显示出高灵敏度和选择性，但荧光探针是复杂的。当液体被超声辐照时，超声空化发生，并且包括微泡的形成、生长和爆炸性破裂。在这项研究中，我们发现超声空化加速聚乙烯亚胺-聚多巴胺荧光纳米颗粒（PEI-PDA NPs）的形成，从而提高灵敏度和减少DA检测的反应时间。将全氟己烷纳米液滴作为外源空化核加入反应体系中，以增强空化效应。在空化过程中，水分子在高温作用下分解为羟基自由基和氢自由基。羟自由基加速DA的氧化，使其聚合成PDA。然后PDA与PEI反应并形成PEI-PDA NP。超声空化制备的PEI-PDA纳米粒的荧光强度高于未经超声处理的样品。增强的荧光强度导致DA检测的灵敏度增加，并且由于加速反应而缩短了时间。在优化条件下，525 nm处的荧光强度与DA浓度在0.1 ~ 100 μM范围内呈良好的线性关系。检测限为25 nM。当10倍干扰物质与DA混合时，该方法也显示出良好的选择性。在脑脊液中进行的进一步实验表明，可接受的准确性和重现性。
Detection of dopamine (DA) is critical to medical diagnosis, and most of existing methods require sophisticated instruments and long processing time. Although fluorimetric methods show high sensitivity and selectivity, fluorescent probes are complicated. Ultrasound cavitation occurs when liquids are irradiated with ultrasound and comprises the formation, growth, and explosive collapse of microbubbles. In this study, we found that ultrasound cavitation accelerated the formation of polyethyleneimine-polydopamine fluorescent nanoparticles (PEI-PDA NPs), thereby enhancing the sensitivity and reducing the reaction time of DA detection. Perfluorohexane nanodroplets served as exogenous cavitation nuclei and were added into the reaction system to enhance the cavitation effect. During cavitation, water molecular was split into hydroxyl and hydrogen free radicals because of the high temperature. Hydroxyl free radicals accelerated the oxidation of DA, which polymerized into PDA. PDA then reacted with PEI and formed PEI-PDA NPs. The fluorescence intensity of PEI-PDA NPs made with ultrasound cavitation was higher than that of samples treated without ultrasound. The enhanced fluorescent intensity resulted in increased sensitivity of DA detection and less time due to accelerated reaction. Under the optimized conditions, the fluorescence intensity at 525 nm and DA concentrations exhibited good linear relationship within 0.1–100 μM. The detection limit was 25 nM. The method also showed excellent selectivity when 10 times interfering chemicals were mixed with DA. Further experiments carried out in cerebrospinal fluid indicated acceptable accuracy and reproducibility.
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影响因子：
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DOI：
10.1002/9783527809080.cataz15486
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期刊：
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