Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley.
Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley.
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DOI：
10.1038/s41467-022-33300-1
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发表时间：
2022-10-13
影响因子：
16.6
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中科院分区：
文献类型：
--
作者：
面对陆地威胁，陆地植物用富含脂质的角质层密封其空中表面。为了呼吸，植物用可调节的表皮孔（称为气孔）打断其角质层，这些孔调节气体交换，并发育其他特殊的表皮细胞，例如防御性毛发。协调表皮特征的机制仍然知之甚少。为了解决这个问题，我们研究了两个等位基因变异导致大麦角质层蜡质缺乏和气孔排列错误的基因座，确定了潜在的基因Cer-g/HvYDA1，编码YODA样（YDA）MAPKKK，以及Cer-s/HvBRX-Solo，编码单个BREVIS-RADIX（BRX）结构域蛋白。这两个基因都控制着表皮的完整性、表皮细胞的间距和特性、大麦独特的表皮蜡华，以及二氧化碳浓度升高条件下的气孔模式。遗传分析揭示了 HvYDA1 和 HvBRX-Solo 之间的上位和修饰关系，表明它们的产物参与连接大麦表皮图案与角质层特性的相互作用途径。这可能代表了一种协调栽培谷物中陆地植物表皮的多种适应性特征的机制。叶子表皮被富含脂质的角质层密封，以防止水分流失，并散布着气孔以进行气体交换。在这里，作者提供了证据，证明两种大麦蛋白 HvYDA1 和 HvBRX-Solo 调节谷类作物中表皮图案与角质层特性之间的两个过程。
Faced with terrestrial threats, land plants seal their aerial surfaces with a lipid-rich cuticle. To breathe, plants interrupt their cuticles with adjustable epidermal pores, called stomata, that regulate gas exchange, and develop other specialised epidermal cells such as defensive hairs. Mechanisms coordinating epidermal features remain poorly understood. Addressing this, we studied two loci whose allelic variation causes both cuticular wax-deficiency and misarranged stomata in barley, identifying the underlying genes, Cer-g/ HvYDA1, encoding a YODA-like (YDA) MAPKKK, and Cer-s/ HvBRX-Solo, encoding a single BREVIS-RADIX (BRX) domain protein. Both genes control cuticular integrity, the spacing and identity of epidermal cells, and barley’s distinctive epicuticular wax blooms, as well as stomatal patterning in elevated CO2 conditions. Genetic analyses revealed epistatic and modifying relationships between HvYDA1 and HvBRX-Solo, intimating that their products participate in interacting pathway(s) linking epidermal patterning with cuticular properties in barley. This may represent a mechanism for coordinating multiple adaptive features of the land plant epidermis in a cultivated cereal. The leaf epidermis is sealed by a lipid-rich cuticle to prevent water loss and interspersed with stomatal pores to allow gas exchange. Here the authors provide evidence that two barley proteins, HvYDA1 and HvBRX-Solo, regulate both processes linking epidermal patterning with cuticular properties in a cereal crop.
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影响因子：
5.6
作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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