Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography.
Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography.
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DOI：
10.1016/j.isci.2023.107210
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发表时间：
2023-07-21
期刊：
影响因子：
5.8
通讯作者：
Ghosal, Debnath
中科院分区：
文献类型：
--
作者：
Kaplan, Doulin C.;Kaplan, Mohammed;Vankadari, Naveen;Kim, Ki Woo;Larson, Charles L.;Dutak, Przemysla;Beare, Paul A.;Krzymowski, Edward;Heinzen, Robert A.;Jensen, Grant J.;Ghosal, Debnath
贝氏柯克斯体是一种专性人畜共患细菌，靶向巨噬细胞，引起一种称为Q热的疾病。它具有双相发育生命周期，其中细胞外和代谢不活跃的小细胞变体（SCV）在宿主体内转化为营养大细胞变体（LCV）。然而，关于这一转变的形态和结构变化的细节仍然缺乏。在这里，我们使用冷冻电子断层扫描成像的SCV和LCV的变种，无论是在无菌条件下生长或直接从宿主细胞纯化。我们发现，SCVs的特点是由内膜的等距堆叠，大概有利于过渡到LCV，过渡耦合的点/Icm型IVB分泌系统（T4 BSS）的表达。一类T4 BSS颗粒与可能参与宿主感染的细胞外密度相关。此外，SCV含有不同大小和位置的球形多层膜结构，表明与孢子形成没有联系。贝氏柯克斯体细胞发育阶段的冷冻电子断层成像揭示贝氏柯克斯体IV型分泌系统的大分子结构贝氏柯克斯体小细胞和大细胞变异体的超微结构特征微生物学;结构生物学
Coxiella burnetii is an obligate zoonotic bacterium that targets macrophages causing a disease called Q fever. It has a biphasic developmental life cycle where the extracellular and metabolically inactive small cell variant (SCV) transforms inside the host into the vegetative large cell variant (LCV). However, details about the morphological and structural changes of this transition are still lacking. Here, we used cryo-electron tomography to image both SCV and LCV variants grown either under axenic conditions or purified directly from host cells. We show that SCVs are characterized by equidistant stacks of inner membrane that presumably facilitate the transition to LCV, a transition coupled with the expression of the Dot/Icm type IVB secretion system (T4BSS). A class of T4BSS particles were associated with extracellular densities possibly involved in host infection. Also, SCVs contained spherical multilayered membrane structures of different sizes and locations suggesting no connection to sporulation as once assumed. Imaging developmental stages of Coxiella burnetii cells using cryo-electron tomography Revealing the macromolecular structure of C. burnetii type IV secretion system The ultrastructural features of the C. burnetii small and large cell variants Microbiology; Structural biology
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影响因子：
64.5
作者：
Costa TRD;Ilangovan A;Ukleja M;Redzej A;Santini JM;Smith TK;Egelman EH;Waksman G
通讯作者：
Waksman G
影响因子：
2.2
作者：
Guertler, Lutz;Bauerfeind, Ursula;Willkommen, Hannelore
通讯作者：
Willkommen, Hannelore
影响因子：
5.6
作者：
EGELMAN, EH;STASIAK, A
通讯作者：
STASIAK, A
影响因子：
3.4
作者：
Graham JG;MacDonald LJ;Hussain SK;Sharma UM;Kurten RC;Voth DE
通讯作者：
Voth DE
影响因子：
3.1
作者：
Hoover, TA;Culp, DW;Thompson, HA
通讯作者：
Thompson, HA