A phospholipidomic analysis of all defined human plasma lipoproteins.
A phospholipidomic analysis of all defined human plasma lipoproteins.
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DOI：
10.1038/srep00139
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发表时间：
2011
影响因子：
4.6
通讯作者：
Rezaee, Farhad
中科院分区：
文献类型：
--
作者：
Dashti, Monireh;Kulik, Willem;Hoek, Frans;Veerman, Enno C.;Peppelenbosch, Maikel P.;Rezaee, Farhad
由于血浆脂蛋白同时含有蛋白质和磷脂成分，两者都可能参与动脉粥样硬化等过程。在这项研究中，进行了血浆脂蛋白相关磷脂的鉴定，这对于在分子水平上理解这些过程是必不可少的。LC-ESI/MS、LC-ESI-MS/MS和高效薄层色谱(HPTLC)分析表明，磷脂酰乙醇胺(PE)、磷脂酰肌醇(PI)和鞘磷脂(SM)仅存在于脂蛋白和磷脂酰胆碱(PC)上，溶血磷脂酰胆碱(LYSO-PC)存在于脂蛋白和血浆脂蛋白无蛋白部分(PLFF)上。心磷脂、磷脂酰甘油(PG)和磷脂酰丝氨酸(PS)在脂蛋白组分和PLFF中均未观察到。所有三种方法都导致了关于血浆脂蛋白和PLFF中出现磷脂的相同结果。极低密度脂蛋白和低密度脂蛋白组分中PE和SM含量均较高。这项研究首次提供了关于所有已定义的血浆脂蛋白的磷脂组成的知识。
Since plasma lipoproteins contain both protein and phospholipid components, either may be involved in processes such as atherosclerosis. In this study the identification of plasma lipoprotein-associated phospholipids, which is essential for understanding these processes at the molecular level, are performed. LC-ESI/MS, LC-ESI-MS/MS and High Performance Thin Layer Chromatography (HPTLC) analysis of different lipoprotein fractions collected from pooled plasma revealed the presence of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and sphingomyeline (SM) only on lipoproteins and phosphatidylcholine (PC), Lyso-PC on both lipoproteins and plasma lipoprotein free fraction (PLFF). Cardiolipin, phosphatidylglycerol (PG) and Phosphatidylserine (PS) were observed neither in the lipoprotein fractions nor in PLFF. All three approaches led to the same results regarding phospholipids occurrence in plasma lipoproteins and PLFF. A high abundancy of PE and SM was observed in VLDL and LDL fractions respectively. This study provides for the first time the knowledge about the phospholipid composition of all defined plasma lipoproteins.
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DOI：
10.1073/pnas.97.4.1743
发表时间：
2000-02-15
影响因子：
11.1
作者：
Deguchi, H;Fernández, JA;Griffin, JH
通讯作者：
Griffin, JH
影响因子：
2.9
作者：
IBDAH, JA;LUNDKATZ, S;PHILLIPS, MC
通讯作者：
PHILLIPS, MC
DOI：
10.1161/01.atv.20.12.2614
发表时间：
2000-12-01
影响因子：
8.7
作者：
Jiang, XC;Paultre, F;Tall, AR
通讯作者：
Tall, AR
影响因子：
5.3
作者：
BAGDADE, JD;BUCHANAN, WF;LITHELL, H
通讯作者：
LITHELL, H
影响因子：
120.7
作者：
CASTELLI, WP;GARRISON, RJ;KANNEL, WB
通讯作者：
KANNEL, WB