Immune function of an angiotensin-converting enzyme against Rice stripe virus infection in a vector insect

Immune function of an angiotensin-converting enzyme against Rice stripe virus infection in a vector insect
复制标题

血管紧张素转换酶对媒介昆虫水稻条纹病毒感染的免疫功能

DOI:
10.1016/j.virol.2019.05.007
复制
发表时间:
2019-05
期刊:
影响因子:
3.7
通讯作者:
Qiao Luqin
Qiao Luqin
中科院分区:
医学3区
文献类型:
--
作者:
Wang Xue;Wang Wei;Zhang Wenzhong;Li Jing;Cui Feng;Qiao Luqin

文献摘要

参考文献

相似文献

血管紧张素转换酶(ACE)在动物界中起着广泛的作用。然而,ACE是否在媒介昆虫中发挥抵抗病毒感染的免疫功能尚不清楚。本研究从褐飞虱体内分离到一个ACE基因(LsACE),该基因对水稻条纹病毒(RSV)的感染有应答作用。在不同pH和温度条件下对LsACE的酶活性进行了表征。二十稻飞虱蛋白质被发现与LsACE相互作用。RSV感染显著上调睾丸和脂肪体中LsACE的表达。当LsACE在含病毒稻飞虱中的表达被抑制时,RSVSP基因的RNA水平在稻飞虱中上调2倍,所有RSV基因在被这些稻飞虱取食的水稻植株中均显示出较高的RNA水平,导致较高的病毒感染率和疾病等级指数。这些结果表明LsACE在对抗稻飞虱RSV传播的免疫应答中起作用。
Angiotensin-converting enzyme (ACE) plays diverse roles in the animal kingdom. However, whether ACE plays an immune function against viral infection in vector insects is unclear. In this study, anACEgene (LsACE) from the small brown planthopper was found to respond toRice stripe virus(RSV) infection. The enzymatic activities of LsACE were characterized at different pH and temperature. Twenty planthopper proteins were found to interact with LsACE. RSV infection significantly upregulatedLsACEexpression in the testicle and fat body. When the expression ofLsACEin viruliferous planthoppers was inhibited, the RNA level of the RSVSPgene was upregulated 2-fold in planthoppers, and all RSV genes showed higher RNA levels in the rice plants consumed by these planthoppers, leading to a higher viral infection rate and disease rating index. These results indicate that LsACE plays a role in the immune response against RSV transmission by planthoppers.
DOI: 10.1007/s12192-013-0407-1
发表时间: 2013-09
影响因子: 3.8
作者:
Kotlarz, Agnieszka;Tukaj, Stefan;Krzewski, Konrad;Brycka, Elzbieta;Lipinska, Barbara
通讯作者: Lipinska, Barbara
水稻条纹病毒在媒介昆虫和宿主植物中的独特复制和基因表达策略
DOI: 10.1099/jgv.0.001255
发表时间: 2019
影响因子: 3.8
作者:
Zhao Wan;Wang Qianshuo;Xu Zhongtian;Liu Renyi;Qui Feng
通讯作者: Qui Feng
DOI: 10.1128/jvi.01504-10
发表时间: 2011-03-01
影响因子: 5.4
作者:
Cilia, M.;Tamborindeguy, C.;Gray, S.
通讯作者: Gray, S.
DOI: 10.1007/s00109-012-0894-z
发表时间: 2012-05
影响因子: 4.7
作者:
Fournier, David;Luft, Friedrich C.;Bader, Michael;Ganten, Detlev;Andrade-Navarro, Miguel A.
通讯作者: Andrade-Navarro, Miguel A.
DOI: 10.5423/ppj.rw.10.2012.0158
发表时间: 2013-09
期刊: The plant pathology journal
影响因子: --
作者:
Cho WK;Lian S;Kim SM;Park SH;Kim KH
通讯作者: Kim KH