MALDI-TOF MS combined with magnetic beads for detecting serum protein biomarkers and establishment of boosting decision tree model for diagnosis of colorectal cancer.
MALDI-TOF MS combined with magnetic beads for detecting serum protein biomarkers and establishment of boosting decision tree model for diagnosis of colorectal cancer.
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DOI：
10.7150/ijms.8.39
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发表时间：
2011-01-03
影响因子：
3.6
通讯作者：
Yong L
中科院分区：
文献类型：
--
作者：
Liu C;Pan C;Shen J;Wang H;Yong L
本研究旨在利用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）技术研究结直肠癌（CRC）患者的血清蛋白指纹图谱，筛选结直肠癌发病和发展过程中与结直肠癌密切相关的蛋白分子。本研究采用144例结直肠癌患者和120名健康志愿者的血清样本。使用弱阳离子交换（WCX）磁珠和PBSII-C蛋白芯片读取器（Ciphergen Biosystems Ins.）。利用Biomarker Wizard系统分析所有血清样品的蛋白指纹图谱表达及癌组和正常组的蛋白指纹图谱。检测到多个蛋白质组学峰，鉴定出4个具有不同表达谱的潜在生物标志物，相对分子量分别为2870.7Da、3084Da、9180.5Da和13748.8Da。4种蛋白中，m/z 2870.7和3084蛋白下调，m/z 9180.5和13748.8蛋白上调。该诊断模型可将结直肠癌与健康对照区分开，敏感性为92.85%，特异性为91.25%。盲测数据显示灵敏度为86.95%，特异性为85%。结果提示MALDI技术可用于筛选结直肠癌患者血清中差异表达的关键蛋白。这些差异调节蛋白被认为是血清中结直肠癌患者的潜在生物标志物，具有进一步研究的潜在价值。
The aim of present study is to study the serum protein fingerprint of patients with colorectal cancer (CRC) and to screen protein molecules that are closely related to colorectal cancer during the onset and progression of the disease with Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Serum samples from 144 patients with CRC and 120 healthy volunteers were adopted in present study. Weak cation exchange (WCX) magnetic beads and PBSII-C protein chips reader (Ciphergen Biosystems Ins.) were used. The protein fingerprint expression of all the Serum samples and the resulted profiles between cancer and normal groups were analyzed with Biomarker Wizard system. Several proteomic peaks were detected and four potential biomarkers with different expression profiles were identified with their relative molecular weights of 2870.7Da, 3084Da, 9180.5Da, and 13748.8Da, respectively. Among the four proteins, two proteins with m/z 2870.7 and 3084 were down-regulated, and the other two with m/z 9180.5 and 13748.8 were up-regulated in serum samples from CRC patients. The present diagnostic model could distinguish CRC from healthy controls with the sensitivity of 92.85% and the specificity of 91.25%. Blind test data indicated a sensitivity of 86.95% and a specificity of 85%. The result suggested that MALDI technology could be used to screen critical proteins with differential expression in the serum of CRC patients. These differentially regulated proteins were considered as potential biomarkers for the patients with CRC in the serum and of the potential value for further investigation.
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癌组学对癌症生物标志物发现的贡献。
影响因子：
37.3
作者：
Cho, William C S
通讯作者：
Cho, William C S
影响因子：
2.8
作者：
BARILLARI, P;RAMACCIATO, G;FEGIZ, G
通讯作者：
FEGIZ, G
DOI：
10.1073/pnas.2033602100
发表时间：
2003-10-14
影响因子：
11.1
作者：
Kozak, KR;Amneus, MW;Farias-Eisner, R
通讯作者：
Farias-Eisner, R
影响因子：
29.4
作者：
Ahlquist, DA;Skoletsky, JE;Shuber, AP
通讯作者：
Shuber, AP
影响因子：
7
作者：
Ma, Yanlei;Peng, Jiayuan;Qin, Huanlong
通讯作者：
Qin, Huanlong