Accumulation of leukotriene C4 and histamine in human allergic skin reactions.
Accumulation of leukotriene C4 and histamine in human allergic skin reactions.
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白三烯 C4 和组胺在人体过敏性皮肤反应中的积累。
DOI：
10.1172/jci112018
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Zweiman,B
中科院分区：
文献类型：
--
作者：
Talbot,SF;Atkins,PC;Goetzl,EJ;Zweiman,B
为了确定花生四烯酸代谢的脂氧合酶产物是否在人体皮肤过敏反应中在体内释放，我们使用放射免疫法和高效液相色谱法分离了放置在剥落皮肤部位的腔液，以检测C-6肽白三烯（如LTC4、LTD4和LTE4）和白三烯B4 （LTB4）的存在，并将其与13名特应性和2名非特应性志愿者的组胺（放射酶法测定）进行了比较。豚草或草花粉抗原（250-750蛋白氮单位/ml）攻毒第1小时，磷酸盐缓冲盐水（PBS）攻毒后3小时，每小时检测一次皮肤腔部位，并与单独PBS攻毒部位进行比较。高效液相色谱法测定，在皮肤部位释放的C-6肽白三烯中，LTC4占85%以上，而LTD4和LTE4的含量很少。在4小时内，LTC4在抗原位点的浓度明显高于pbs刺激位点。在这段时间内发现了最低浓度的LTB4，在抗原或PBS位点没有差异。组胺在抗原位点的浓度明显高于PBS位点，但释放模式与LTC4不同。组胺释放高峰总是出现在第一个小时，随后逐渐下降，而LTC4的最大量在第2至第4小时被检测到。在该部位积累的LTC4的量取决于表皮刺激中使用的抗原的剂量。我们在这项研究中已经证明，在评估的白三烯中，LTC4在体内皮肤过敏性反应中就地释放量最大，并且在抗原激发后至少4小时存在于这些部位。由于人类皮内注射LTC4可诱导持续数小时的轮状和耀斑反应，我们的研究结果支持LTC4是人类皮肤过敏性反应重要介质的假设。
To determine whether lipoxygenase products of arachidonic acid metabolism are released in vivo during human allergic cutaneous reactions, we serially assayed chamber fluid placed over denuded skin sites for the presence of both C-6 peptide leukotrienes (e.g., LTC4, LTD4, and LTE4) and leukotriene B4 (LTB4), using radioimmune assay and HPLC separation, and compared it to histamine (assayed radioenzymatically) in 13 atopic and two nonatopic volunteers. Skin chamber sites challenged with ragweed or grass pollen antigen (250-750 protein nitrogen units/ml) for the first hour and phosphate-buffered saline (PBS) for the next 3 h were assayed hourly and compared to sites challenged with PBS alone. As assessed by HPLC, LTC4 composed greater than 85% of the C-6 peptide leukotriene released at any skin site, whereas little LTD4 or LTE4 was detected. LTC4 was present in significantly greater concentrations at antigen sites as compared to PBS-challenged sites throughout the 4-h period. Minimal concentrations of LTB4 were found throughout this time period and were not different at antigen or PBS sites. Histamine was present in significantly greater concentrations at antigen rather than PBS sites, but the pattern of release was different from that of LTC4. Peak histamine release invariably occurred during the first hour and decreased progressively thereafter, whereas the greatest amounts of LTC4 were detected during the 2nd to 4th hours. The amount of LTC4 accumulating at the site was dependent upon the dosage of antigen used in the epicutaneous challenge. We have demonstrated in this study that of the leukotrienes assessed LTC4 is released in the greatest quantity in situ during in vivo allergic cutaneous reactions and that it is present at such sites for at least 4 h after antigen challenge. Since intradermal injection of LTC4 in humans induces wheal and flare responses that persist for hours, our findings support the hypothesis that LTC4 is an important mediator of human allergic skin reactions.
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白三烯 C 和 D 对人体产生的支气管收缩
DOI：
--
发表时间：
1981
期刊：
The Lancet
影响因子：
--
作者：
M. Holroyde;M. Cole;R. Altounyan;M. Dixon;E. V. Elliott
通讯作者：
E. V. Elliott
影响因子：
2.9
作者：
BISGAARD, H;FORDHUTCHINSON, AW;TAUDORF, E
通讯作者：
TAUDORF, E
影响因子：
2.9
作者：
BISGAARD, H;KRISTENSEN, J;SONDERGAARD, J
通讯作者：
SONDERGAARD, J
通过放射酶测定法测量组胺的血浆浓度：组胺酶孵育的影响。
DOI：
10.1016/0091-6749(82)90085-9
发表时间：
1982
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Atkins,PC;Valenzano,M;Zweiman,B
通讯作者：
Zweiman,B
白三烯 C4 的灵敏且特异的放射免疫测定法
影响因子：
3.5
作者：
J. Lindgren;S. Hammerström;E. Goetzl
通讯作者：
E. Goetzl