Comparative microRNA-seq Analysis Depicts Candidate miRNAs Involved in Skin Color Differentiation in Red Tilapia.
Comparative microRNA-seq Analysis Depicts Candidate miRNAs Involved in Skin Color Differentiation in Red Tilapia.
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DOI：
10.3390/ijms19041209
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发表时间：
2018-04-16
影响因子：
5.6
通讯作者：
Fu J
中科院分区：
文献类型：
--
作者：
Wang L;Zhu W;Dong Z;Song F;Dong J;Fu J
红罗非鱼体色的分化和变异越来越限制其商品价值。迄今为止，有关鱼类肤色分化和变异的microRNA（miRNA）信息有限。在这项研究中，对三种颜色的红罗非鱼进行了高通量Illumina sRNA测序，产生了81，394，491个原始读数。共158个差异表达的miRNAs（|log 2（倍数变化）|≥ 1且q值≤ 0.001）。颜色相关miRNAs的靶基因预测和功能分析表明，多种推定的靶基因，包括slc 7a 11，mc 1 r和asip，在色素沉着中发挥了潜在的作用。此外;通过构建miRNA-mRNA调控网络，阐明了miR-138- 5 p和miR-722在色素形成过程中的重要作用。这些结果促进了我们对红罗非鱼皮肤色素分化的分子机制的理解。
Differentiation and variation in body color has been a growing limitation to the commercial value of red tilapia. Limited microRNA (miRNA) information is available on skin color differentiation and variation in fish so far. In this study, a high-throughput Illumina sequencing of sRNAs was conducted on three color varieties of red tilapia and 81,394,491 raw reads were generated. A total of 158 differentially expressed miRNAs (|log2(fold change)| ≥ 1 and q-value ≤ 0.001) were identified. Target prediction and functional analysis of color-related miRNAs showed that a variety of putative target genes—including slc7a11, mc1r and asip—played potential roles in pigmentation. Moreover; the miRNA-mRNA regulatory network was illustrated to elucidate the pigmentation differentiation, in which miR-138-5p and miR-722 were predicted to play important roles in regulating the pigmentation process. These results advance our understanding of the molecular mechanisms of skin pigmentation differentiation in red tilapia.
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影响因子：
14.9
作者：
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通讯作者：
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DOI：
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