A series of protein phosphatase gene disruptants in Saccharomyces cerevisiae
A series of protein phosphatase gene disruptants in Saccharomyces cerevisiae
复制标题
酿酒酵母中一系列蛋白磷酸酶基因破坏子
作者：
N. Sakumoto;Y. Mukai;Kouji Uchida;T. Kouchi;Jyoh Kuwajima;Y. Nakagawa;Shigemi Sugioka;Eishi Yamamoto;Tomomi Furuyama;Hiroyuki Mizubuchi;Naoshi Ohsugi;T. Sakuno;K. Kikuchi;Itsumi Matsuoka;N. Ogawa;Y. Kaneko;S. Harashima
在酿酒酵母的基因组核苷酸序列中鉴定了32个蛋白磷酸酶（PPase）基因。我们构造了S。酿酒酵母破坏剂的PPase基因的每一个，并检查它们在各种条件下的生长。本研究首次创建了YBR 125 c、YCR 079 w、YIL 113 w、YJR 110 w、YNR 022 c和YOR 090 c六个推定PPase基因的破坏子。glc7、sit4和cdc14破坏剂在我们的菌株背景中是致死的。其余29个PPase基因破坏物在30°C和37°C下都能存活，但只有一个破坏物yvh 1在13°C下表现出内在的冷敏感性生长。YVH1基因的转录在13°C下被诱导，这与Yvh1p在低温下生长具有特定作用的想法一致。在以蔗糖、半乳糖、麦芽糖或甘油为碳源的培养基上，能正常生长。PPZ 1干扰物耐受NaCl和LiCl，而cmp 2干扰物对这些盐敏感，如先前所报道的，并且没有其他可行的PPase干扰物表现出盐敏感性。当测试活性干扰物对药物（即苯菌灵、咖啡因和羟基脲）的敏感性时，ppz1和ycr 079w干扰物表现出对咖啡因的敏感性。版权所有© 1999年约翰威利父子有限公司。
Thirty‐two protein phosphatase (PPase) genes were identified in the genome nucleotide sequence of Saccharomyces cerevisiae. We constructed S. cerevisiae disruptants for each of the PPase genes and examined their growth under various conditions. The disruptants of six putative PPase genes, i.e. of YBR125c, YCR079w, YIL113w, YJR110w, YNR022c and YOR090c, were created for the first time in this study. The glc7, sit4 and cdc14 disruptants were lethal in our strain background. The remaining 29 PPase gene disruptants were viable at 30°C and 37°C, but only one disruptant, yvh1, showed intrinsic cold‐sensitive growth at 13°C. Transcription of the YVH1 gene was induced at 13°C, consistent with an idea that Yvh1p has a specific role for growth at a low temperature. The viable disruptants grew normally on nutrient medium containing sucrose, galactose, maltose or glycerol as carbon sources. The ppz1 disruptant was tolerant to NaCl and LiCl, while the cmp2 disruptant was sensitive to these salts, as reported previously, and none of the other viable PPase disruptants exhibited the salt sensitivity. When the viable disruptants were tested for sensitivity to drugs, i.e. benomyl, caffeine and hydroxyurea, ppz1 and ycr079w disruptants exhibited sensitivity to caffeine. Copyright © 1999 John Wiley & Sons, Ltd.
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DOI：
10.1016/s0021-9258(19)49907-9
发表时间：
1992-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Wan;H. Xu;M. Grunstein
通讯作者：
J. Wan;H. Xu;M. Grunstein
影响因子：
3.3
作者：
Sia, RAL;Herald, HA;Lew, DJ
通讯作者：
Lew, DJ
DOI：
10.1073/pnas.89.24.12175
发表时间：
1992-12-15
影响因子：
11.1
作者：
GUAN, K;HAKES, DJ;DIXON, JE
通讯作者：
DIXON, JE
影响因子：
3.3
作者：
J. Cannon;J. Pringle;A. Fiechter;M. Khalil
通讯作者：
J. Cannon;J. Pringle;A. Fiechter;M. Khalil
Glc7p 蛋白磷酸酶调节酵母糖原代谢和孢子形成。
影响因子：
3.3
作者：
Ramaswamy,NT;Li,L;Khalil,M;Cannon,JF
通讯作者：
Cannon,JF