Intergenerational and parent of origin effects of maternal calorie restriction on Igf2 expression in the adult rat hippocampus.
Intergenerational and parent of origin effects of maternal calorie restriction on Igf2 expression in the adult rat hippocampus.
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DOI：
10.1016/j.psyneuen.2014.04.002
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发表时间：
2014-07
影响因子：
3.7
通讯作者：
Redei, Eva E.
中科院分区：
文献类型：
--
作者：
Harper, Kathryn M.;Tunc-Ozcan, Elif;Graf, Evan N.;Herzing, Laura B. K.;Redei, Eva E.
关键词：
胰岛素样生长因子2（Igf 2）调节海马中的发育、记忆和成人神经发生。热量限制（CR）是已知的，以调节非神经元Igf 2的表达代际，但其影响尚未评估脑Igf 2。在此，Sprague-Dawley（S）母鼠在妊娠第8-21天发生中度CR。为了鉴定印迹Igf 2基因的原始表达模式的亲本，将其后代（SS F1）与未处理的雄性或雌性Brown Norway（B）大鼠交配，以获得第二代（BS和SB F2）后代。CR不影响SS F1雄性或其BS F2后代的成年海马Igf 2转录水平，但增加SS F1雌性及其SB F2后代的水平。SB F2对照雄性海马中的优先母体Igf 2表达随着CR而放松至双等位基因，在任何其他组中均不存在祖母饮食的影响。因此，海马Igf 2的等位基因特异性和总表达受母体、祖母体CR的影响，且具有应变和性别特异性。
Insulin-like growth factor 2 (Igf2) regulates development, memory and adult neurogenesis in the hippocampus. Calorie restriction (CR) is known to modulate non-neuronal Igf2 expression intergenerationally, but its effect has not been evaluated on brain Igf2. Here, Sprague-Dawley (S) dams underwent moderate CR between gestational days 8–21. To identify parent of origin expression pattern of the imprinted Igf2 gene, their offspring (SS F1) were mated with naïve male or female Brown Norway (B) rats to obtain the second generation (BS and SB F2) progeny. CR did not affect adult hippocampal Igf2 transcript levels in SS F1 males or their BS F2 progeny, but increased it in SS F1 females and their SB F2 offspring. The preferentially maternal Igf2 expression in the SB F2 control male hippocampus relaxed to biallelic with CR, with no effect of grandmaternal diet in any other groups. Thus, allele-specific and total expression of hippocampal Igf2 is affected by maternal, grandmaternal CR in a strain and sex-specific manner.
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影响因子：
4.6
作者：
Harper, Kathryn M.;Tunc-Ozcan, Elif;Redei, Eva E.
通讯作者：
Redei, Eva E.
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影响因子：
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影响因子：
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