Arabidopsis thaliana Yellow Stripe1-Like4 and Yellow Stripe1-Like6 localize to internal cellular membranes and are involved in metal ion homeostasis.
Arabidopsis thaliana Yellow Stripe1-Like4 and Yellow Stripe1-Like6 localize to internal cellular membranes and are involved in metal ion homeostasis.
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DOI：
10.3389/fpls.2013.00283
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发表时间：
2013
影响因子：
5.6
通讯作者：
Walker EL
中科院分区：
文献类型：
--
作者：
Conte SS;Chu HH;Rodriguez DC;Punshon T;Vasques KA;Salt DE;Walker EL
类黄色条纹1（YSL）转运蛋白家族的若干成员能够转运金属 - 烟酰胺（NA）复合物。在理解与该家族创始成员ZmYS1关系最为密切的拟南芥YSL蛋白（例如AtYSL1、AtYSL2和AtYSL3）的作用方面已经取得了重大进展，但关于另外两个高度保守的YSL分支的成员信息却很少。在此，我们提供证据表明，拟南芥中属于YSL第二组的仅有的两个基因AtYSL4和AtYSL6定位于液泡膜以及类似于内质网的内膜上。对YSL4和YSL6的单突变体和双突变体进行了严格分析，尽管YSL6表达强烈且广泛，但它们却具有令人惊讶的轻微表型。然而，在锰和镍达到毒性水平时，YSL4和YSL6发生突变的植株以及过表达带有绿色荧光蛋白标签的YSL6的植株都表现出生长缺陷，这表明这些转运蛋白在重金属胁迫响应中具有作用。
Several members of the Yellow Stripe1-Like (YSL) family of transporter proteins are able to transport metal-nicotianamine (NA) complexes. Substantial progress has been made in understanding the roles of the Arabidopsis YSLs that are most closely related to the founding member of the family, ZmYS1 (e.g., AtYSL1, AtYSL2 and AtYSL3), but there is little information concerning members of the other two well-conserved YSL clades. Here, we provide evidence that AtYSL4 and AtYSL6, which are the only genes in Arabidopsis belong to YSL Group II, are localized to vacuole membranes and to internal membranes resembling endoplasmic reticulum. Both single and double mutants for YSL4 and YSL6 were rigorously analyzed, and have surprisingly mild phenotypes, in spite of the strong and wide-ranging expression of YSL6. However, in the presence of toxic levels of Mn and Ni, plants with mutations in YSL4 and YSL6 and plants overexpressing GFP-tagged YSL6 showed growth defects, indicating a role for these transporters in heavy metal stress responses.
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影响因子：
11.6
作者：
Divol, Fanchon;Couch, Daniel;Curie, Catherine
通讯作者：
Curie, Catherine
影响因子：
4.9
作者：
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作者：
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通讯作者：
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影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
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影响因子：
7.2
作者：
Arrivault, Stephanie;Senger, Toralf;Kraemer, Ute
通讯作者：
Kraemer, Ute