Effects of heat shock treatment on glucose metabolism in grass carp (Ctenopharyngodon idellus) juveniles
Effects of heat shock treatment on glucose metabolism in grass carp (Ctenopharyngodon idellus) juveniles
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热激处理对草鱼幼鱼糖代谢的影响
DOI：
10.1016/j.aquaculture.2013.12.021
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发表时间：
2014-02
期刊：
影响因子：
4.5
通讯作者：
何焱
中科院分区：
文献类型：
--
作者：
何焱
相对于哺乳动物，鱼类的碳水化合物代谢能力较差。具体来说，鱼不能很好地利用膳食葡萄糖。热休克蛋白72（Heat shock protein 72，Hsp 72）可通过热休克诱导并阻止应激诱导的c-Jun氨基N-末端激酶（JNK）的活化，进而促进哺乳动物的胰岛素信号通路。然而，Hsp 72在鱼类葡萄糖利用中的功能尚不清楚。以草鱼幼鱼为材料，研究了热激对草鱼葡萄糖利用的影响。本研究采用高糖饲料和对照饲料饲喂草鱼幼鱼。一半的青少年在8周内进行了几次临时热休克治疗。分析各组幼鱼的生长性能、体成分和肝脏中包括Hsp 72在内的糖代谢相关基因的表达。热激处理能提高草鱼幼鱼的生长性能和体成分，并能激活JNK。尽管荧光真实的时间PCR和Western印迹检测显示GH组（高糖饲料喂养并热休克处理的幼鱼）Hsp 72的表达显着增加（P< 0.05），但在我们的研究中，Hsp 72并未抑制JNK和p-JNK的表达，这与之前在哺乳动物中的一些研究不同。综上所述，本研究表明热激并没有通过JNK途径影响草鱼的葡萄糖代谢。这可能为解释为什么鱼类的碳水化合物代谢能力比哺乳动物差得多提供了线索。
Fish species have poor capability of carbohydrate metabolism relative to mammals. Specifically, fish cannot utilize dietary glucose well. Heat shock protein 72 (Hsp72) can be induced by heat shock and prevent stress-induced activation of c-Jun amino N-terminal kinase (JNK) which then promotes the insulin signaling pathway in mammals. However, the function ofHsp72on glucose utilization is unknown in fish. We conducted a study to understand the role played by heat shock on glucose utilization using juveniles of the grass carp fish. In this study, the juveniles of grass carp were fed with either high glucose diet or control diet. Half of the juveniles were subjected to several temporary heat shock treatments within 8 weeks. The growth performance, body composition of juveniles and the expression of glucose metabolism related genes, includingHsp72in the livers of juveniles were analyzed from each group. The heat shock treatments could improve growth performance and body composition of grass carp juveniles, and could activate JNK. Though the expression ofHsp72increased significantly in Group GH (juveniles fed with high-glucose diet and given heat shock treatment) (P< 0.05) as measured by fluorescent real time PCR and Western blot, the expression of JNK and p-JNK were not suppressed by Hsp72 in our study, which was different from some previous studies in mammals. In summary, the study showed that heat shock did not affect glucose metabolism through JNK pathway in grass carp. This may give a clue to explain why fish species have a much poorer capability of carbohydrate metabolism than mammals.
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影响因子：
2.7
作者：
Jurczyk A;Roy N;Bajwa R;Gut P;Lipson K;Yang C;Covassin L;Racki WJ;Rossini AA;Phillips N;Stainier DY;Greiner DL;Brehm MA;Bortell R;diIorio P
通讯作者：
diIorio P
DOI：
--
发表时间：
1979
期刊：
Annales de la nutrition et de l'alimentation
影响因子：
--
作者：
F. Bergot
通讯作者：
F. Bergot
影响因子：
1.9
作者：
Takeshi Watanabe
通讯作者：
Takeshi Watanabe
影响因子：
7.2
作者：
R. Giannarelli;M. Aragona;A. Coppelli;S. D. Prato
通讯作者：
R. Giannarelli;M. Aragona;A. Coppelli;S. D. Prato
DOI：
10.1097/mco.0b013e32830460c2
发表时间：
2008-07-01
影响因子：
3.1
作者：
Navab, Mohamad;Gharavi, Nima;Watson, Andrew D.
通讯作者：
Watson, Andrew D.