Thyroid Hormones Regulate Zebrafish Melanogenesis in a Gender-Specific Manner.
Thyroid Hormones Regulate Zebrafish Melanogenesis in a Gender-Specific Manner.
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DOI：
10.1371/journal.pone.0166152
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Cerdá-Reverter JM
中科院分区：
文献类型：
--
作者：
Guillot R;Muriach B;Rocha A;Rotllant J;Kelsh RN;Cerdá-Reverter JM
用抗甲状腺化合物如苯基硫脲处理斑马鱼胚胎，以抑制黑素生成。然而，甲状腺系统控制黑色素合成的机制尚未详细评估。在这项工作中，我们测试了添加T3（500μg/g食物）的饲料对成年斑马鱼色素模式的影响。口服T3在成年雌性和雄性斑马鱼中均诱导明显的皮肤苍白，停止治疗后可逆。可见的黑色素细胞的数量显着减少处理的鱼。因此，治疗下调酪氨酸酶相关蛋白1在两种性别中的表达。我们还发现了一些黑素生成基因的性二态调节，如Dct/Tyrp 2在T3治疗后的女性中显著上调。因此，我们证明了黑素生成是可逆的抑制甲状腺激素在成年斑马鱼和黑素基因表达的反应中的性别特异性差异的发现。因此，鱼的性别现在被证明是一个重要的变量，应在未来的研究控制鱼类黑素合成。
Zebrafish embryos are treated with anti-thyroidal compounds, such as phenylthiourea, to inhibit melanogenesis. However, the mechanism whereby the thyroidal system controls melanin synthesis has not been assessed in detail. In this work, we tested the effect of the administration of diets supplemented with T3 (500μg/g food) on the pigment pattern of adult zebrafish. Oral T3 induced a pronounced skin paling in both adult female and male zebrafish that was reversible upon cessation of treatment. The number of visible melanophores was significantly reduced in treated fish. Accordingly, treatment down-regulated expression of tyrosinase-related protein 1 in both sexes. We also found sexually dimorphic regulation of some melanogenic genes, such as Dct/Tyrp2 that was dramatically up-regulated in females after T3 treatment. Thus, we demonstrated that melanogenesis is reversibly inhibited by thyroid hormones in adult zebrafish and make the discovery of gender-specific differences in the response of melanogenic gene expression. Thus, fish gender is now shown to be an important variable that should be controlled in future studies of fish melanogenesis.
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影响因子：
3.7
作者：
Darias MJ;Andree KB;Boglino A;Rotllant J;Cerdá-Reverter JM;Estévez A;Gisbert E
通讯作者：
Gisbert E
影响因子：
--
作者：
Josep Agulleiro, Maria;Cortes, Raul;Miguel Cerda-Reverter, Jose
通讯作者：
Miguel Cerda-Reverter, Jose
影响因子：
4.3
作者：
Krauss, Jana;Geiger-Rudolph, Silke;Irion, Uwe
通讯作者：
Irion, Uwe
影响因子：
4.5
作者：
Budi EH;Patterson LB;Parichy DM
通讯作者：
Parichy DM
影响因子：
3
作者：
Karlsson, J;von Hofsten, J;Olsson, PE
通讯作者：
Olsson, PE