Morphological and molecular characterization of dietary-induced pseudo-albinism during post-embryonic development of Solea senegalensis (Kaup, 1858).
Morphological and molecular characterization of dietary-induced pseudo-albinism during post-embryonic development of Solea senegalensis (Kaup, 1858).
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DOI：
10.1371/journal.pone.0068844
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gisbert E
中科院分区：
文献类型：
--
作者：
Darias MJ;Andree KB;Boglino A;Rotllant J;Cerdá-Reverter JM;Estévez A;Gisbert E
从形态和分子水平研究了塞内加尔比目鱼(Solea senegalens，Kaup 1858)发育过程中假白化表型的出现。为了诱导假性白化病的发生，给塞内加尔比目鱼幼虫喂食富含花生四烯酸(ARA)的卤虫。将它们的皮肤色素沉着的发展与对照组进行比较，对照组喂食富含参考商品的卤虫。皮肤黑素载体、黄色素载体和虹膜载体的相对数量表明，两组幼虫的色素沉着发展相似。然而，从不同的皮肤色团的相对比例、分配方式、形状和大小的结果显示，从孵化后33d起，ARA组和对照组之间的色素沉积模式发生了变化。在ARA组中没有形成变态后应该出现的新的发色团群体。此外，已经存在的幼虫黄团和黑素团之间的空间分布模式表明，似乎与这些色团的降解有关的短程相互作用，导致了假白化表型的出现。几个关键的色素沉着相关基因的表达谱显示，在没有足够程度的末端分化的情况下，假白化细胞促进了黑素细胞的发育，从而阻止了黑素的产生。目前的结果提示，asip1和SLC24A5基因可能在trp1表达下调中发挥作用，从而导致黑色素产生缺陷。此外，基因表达数据支持Pax3、MITF和asip1基因参与了新的黑素细胞、黄色素细胞和虹膜细胞变性后种群的发育中断。
The appearance of the pseudo-albino phenotype was investigated in developing Senegalese sole (Solea senegalensis, Kaup 1858) larvae at morphological and molecular levels. In order to induce the development of pseudo-albinos, Senegalese sole larvae were fed Artemia enriched with high levels of arachidonic acid (ARA). The development of their skin pigmentation was compared to that of a control group fed Artemia enriched with a reference commercial product. The relative amount of skin melanophores, xanthophores and iridophores revealed that larval pigmentation developed similarly in both groups. However, results from different relative proportions, allocation patterns, shapes and sizes of skin chromatophores revealed changes in the pigmentation pattern between ARA and control groups from 33 days post hatching onwards. The new populations of chromatophores that should appear at post-metamorphosis were not formed in the ARA group. Further, spatial patterns of distribution between the already present larval xanthophores and melanophores were suggestive of short-range interaction that seemed to be implicated in the degradation of these chromatophores, leading to the appearance of the pseudo-albino phenotype. The expression profile of several key pigmentation-related genes revealed that melanophore development was promoted in pseudo-albinos without a sufficient degree of terminal differentiation, thus preventing melanogenesis. Present results suggest the potential roles of asip1 and slc24a5 genes on the down-regulation of trp1 expression, leading to defects in melanin production. Moreover, gene expression data supports the involvement of pax3, mitf and asip1 genes in the developmental disruption of the new post-metamorphic populations of melanophores, xanthophores and iridophores.
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