Post-embryonic nerve-associated precursors to adult pigment cells: genetic requirements and dynamics of morphogenesis and differentiation.
Post-embryonic nerve-associated precursors to adult pigment cells: genetic requirements and dynamics of morphogenesis and differentiation.
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DOI：
10.1371/journal.pgen.1002044
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发表时间：
2011-05
期刊：
影响因子：
4.5
通讯作者：
Parichy DM
中科院分区：
文献类型：
--
作者：
Budi EH;Patterson LB;Parichy DM
脊椎动物的色素细胞具有多种功能，并产生令人惊叹的各种图案。这些细胞也涉及人类病理学，包括黑色素瘤。然而，色素细胞发育的事件已被广泛研究在胚胎中，少得多的是已知的形态发生和这些细胞在胚胎后阶段的分化。先前对斑马鱼的研究揭示了胚胎和成年黑色素细胞的遗传差异，黑色素细胞是斑马鱼黑色素细胞的外温同源物。在这里，我们使用分子标记，重要的标签，延时成像，突变分析和转基因识别外周神经作为一个利基的前体成年黑色素细胞，随后迁移到皮肤形成成人色素模式。我们进一步确定了建立，维持和招募成年黑色素细胞谱系的前体的遗传要求，并在突变背景下的模式调控过程中表现出新的补偿行为。最后，我们表明，不同的人口的潜在的前体有差异的再生能力持续到成人。这些发现为今后研究胚胎后色素细胞前体在发育、进化和肿瘤形成中的作用奠定了基础。了解后胚胎干细胞和祖细胞的生物学是基础和翻译的重要性。为了确定干细胞和祖细胞的建立、维持和招募机制，我们使用斑马鱼成体色素模式。以前的工作表明，胚胎和成人色素细胞有不同的遗传要求，但对成人色素细胞前体的分子或增殖表型或这些前体在胚胎后发育过程中的位置知之甚少。我们在这里表明，胚胎后色素细胞前体与周围神经，这些细胞迁移到皮肤在幼虫到成人的转变时，成人色素图案的形式。我们还定义了几个基因在促进这些事件的形态发生和分化作用。最后，我们证明了潜在的前体池持续到成人和不同的池有不同的能力，提供新的色素细胞的背景下，图案再生。我们的研究为未来的分析奠定了基础，以确定色素干细胞生物学的其他共同和基本特征。
The pigment cells of vertebrates serve a variety of functions and generate a stunning variety of patterns. These cells are also implicated in human pathologies including melanoma. Whereas the events of pigment cell development have been studied extensively in the embryo, much less is known about morphogenesis and differentiation of these cells during post-embryonic stages. Previous studies of zebrafish revealed genetically distinct populations of embryonic and adult melanophores, the ectotherm homologue of amniote melanocytes. Here, we use molecular markers, vital labeling, time-lapse imaging, mutational analyses, and transgenesis to identify peripheral nerves as a niche for precursors to adult melanophores that subsequently migrate to the skin to form the adult pigment pattern. We further identify genetic requirements for establishing, maintaining, and recruiting precursors to the adult melanophore lineage and demonstrate novel compensatory behaviors during pattern regulation in mutant backgrounds. Finally, we show that distinct populations of latent precursors having differential regenerative capabilities persist into the adult. These findings provide a foundation for future studies of post-embryonic pigment cell precursors in development, evolution, and neoplasia. Understanding the biology of post-embryonic stem and progenitor cells is of both basic and translational importance. To identify mechanisms by which stem and progenitor cells are established, maintained, and recruited to particular fates, we are using the zebrafish adult pigment pattern. Previous work showed that embryonic and adult pigment cells have different genetic requirements, but little is known about the molecular or proliferative phenotypes of precursors to adult pigment cells or where these precursors reside during post-embryonic development. We show here that post-embryonic pigment cell precursors are associated with peripheral nerves and that these cells migrate to the skin during the larval-to-adult transformation when the adult pigment pattern forms. We also define morphogenetic and differentiative roles for several genes in promoting these events. Finally, we demonstrate that latent precursor pools persist into the adult and that different pools have different capacities for supplying new pigment cells in the context of pattern regeneration. Our study sets the stage for future analyses to identify additional common and essential features of pigment stem cell biology.
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影响因子：
4.6
作者：
Greco, Valentina;Guo, Shangqin
通讯作者：
Guo, Shangqin
DOI：
10.1073/pnas.0831229100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Dupin, E;Real, C;Le Douarin, NM
通讯作者：
Le Douarin, NM
影响因子：
64.5
作者：
Adameyko, Igor;Lallemend, Francois;Ernfors, Patrik
通讯作者：
Ernfors, Patrik
DOI：
10.1073/pnas.97.14.7882
发表时间：
2000-07-05
影响因子：
11.1
作者：
Dupin, E;Glavieux, C;Le Douarin, NM
通讯作者：
Le Douarin, NM
影响因子：
2
作者：
Ceol CJ;Houvras Y;White RM;Zon LI
通讯作者：
Zon LI