RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination.
RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination.
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DOI：
10.1016/j.molcel.2008.07.006
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发表时间：
2008-08-08
期刊：
影响因子：
16
通讯作者：
Hunter, Neil
中科院分区：
文献类型：
--
作者：
Oh, Steve D.;Lao, Jessica P.;Taylor, Andrew F.;Smith, Gerald R.;Hunter, Neil
酿酒酵母RecQ解旋酶Sgs 1和XPF家族核酸内切酶Mus 81-Mms 4参与加工接合分子（JM）重组中间体。我们表明，缺乏任何一种酶的细胞在减数分裂期间经常遇到染色体分离问题，当两种酶都不存在时，尝试的分离就会灾难性地失败。在所有情况下，隔离似乎都受到未解决的司法措施的阻碍。重组的DNA事件的分析表明，Sgs 1限制异常JM结构，导致从二级链入侵事件，并往往需要Mus 81-Mms 4为他们的正常分辨率。异常JM含有高水平的单霍利迪连接，包括intersister JM，多染色单体JM包括三个和四个染色单体，和新鉴定的重组JM含有两个染色单体，其中一个已经经历了交换。尽管在sgs 1 mms 4双突变体中持续存在JM，但交叉和非交叉产物仍以高水平形成。我们的结论是，Sgs 1和Mus 81-Mms 4合作，以消除异常的JM，而尚未确定的酶处理正常的JM。
Saccharomyces cerevisiae RecQ helicase, Sgs1, and XPF-family endonuclease, Mus81-Mms4, are implicated in processing joint molecule (JM) recombination intermediates. We show that cells lacking either enzyme frequently experience chromosome segregation problems during meiosis and when both enzymes are absent attempted segregation fails catastrophically. In all cases, segregation appears to be impeded by unresolved JMs. Analysis of the DNA events of recombination indicates that Sgs1 limits aberrant JM structures that result from secondary strand-invasion events and often require Mus81-Mms4 for their normal resolution. Aberrant JMs contain high levels of single Holliday junctions and include intersister JMs, multi-chromatid JMs comprising three and four chromatids, and newly identified recombinant JMs containing two chromatids, one of which has undergone crossing-over. Despite persistent JMs in sgs1 mms4 double mutants, crossover and noncrossover products still form at high levels. We conclude that Sgs1 and Mus81-Mms4 collaborate to eliminate aberrant JMs whereas as-yet-unidentified enzymes process normal JMs.
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