Expression of gap-junctional protein (connexin 43 or alpha 1 gap junction) is down-regulated at the transcriptional level during adipocyte differentiation of H-1/A marrow stromal cells.
Expression of gap-junctional protein (connexin 43 or alpha 1 gap junction) is down-regulated at the transcriptional level during adipocyte differentiation of H-1/A marrow stromal cells.
复制标题
在 H-1/A 骨髓基质细胞的脂肪细胞分化过程中，间隙连接蛋白（连接蛋白 43 或 α 1 间隙连接）的表达在转录水平上下调。
作者：
A. Umezawa;J. Hata
骨髓基质细胞是造血细胞增殖所必需的，并通过缝隙连接相互沟通。骨髓基质细胞表达连接蛋白43，但不表达连接蛋白32。小鼠骨髓基质细胞系H-1/A在汇合时经历脂肪细胞分化，并在分化前表达连接蛋白43的3.0倍酶mRNA种类。H-1/A细胞与染料转移法研究表明，间隙连接通信与相邻的细胞，但失去了这种通信在分化过程中。在诱导甘油一磷酸脱氢酶表达之前，H-1/A细胞中的连接蛋白43转录本被下调。缝隙连接通讯的丢失在连接蛋白43的mRNA水平上受到调节。连接蛋白43的表达在转录水平下调。
Bone marrow stromal cells are requisite for the proliferation of hematopoietic cells and communicate with each other via gap junctions. Marrow stromal cells expressed connexin 43, but not connexin 32. H-1/A, a murine marrow stromal cell line, underwent adipocyte differentiation at confluence, and expressed the 3.0 kilobase mRNA species of connexin 43 before differentiation. H-1/A cells studied with the dye-transfer method showed gap-junctional communication with adjacent cells but lost this communication during differentiation. The connexin 43 transcripts in H-1/A cells were down-regulated before the expression of glycero-monophosphate dehydrogenase was induced. Loss of gap-junctional communication was regulated at the mRNA level of connexin 43. Connexin 43 expression was down-regulated at the transcriptional level.
DOI：
10.1073/pnas.78.11.6963
发表时间：
1981
影响因子：
11.1
作者：
Harigaya,K;Cronkite,EP;Miller,ME;Shadduck,RK
通讯作者：
Shadduck,RK
影响因子：
56.9
作者：
TORTI, FM;DIECKMANN, B;RINGOLD, GM
通讯作者：
RINGOLD, GM