SraL sRNA interaction regulates the terminator by preventing premature transcription termination of rho mRNA
SraL sRNA interaction regulates the terminator by preventing premature transcription termination of rho mRNA
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SraL sRNA 相互作用通过防止 rho mRNA 过早转录终止来调节终止子
DOI：
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发表时间：
2019
影响因子：
11.1
通讯作者：
C. Arraiano
中科院分区：
文献类型：
--
作者：
I. J. Silva;Susana Barahona;Alex Eyraud;David Lalaouna;N. Figueroa;É. Massé;C. Arraiano
Rho是一种促进基因组特定区域转录终止的必需蛋白。它的活性不仅在基因的末端很重要，而且在它具有调节功能的前导区域内也很重要。该蛋白质被证明参与rho mRNA的调节，促进其过早的转录终止。在这项研究中，我们在这种蛋白质的复杂调控途径中加入了一个额外的参与者。使用体内和体外实验，显示小的非编码RNA SraL直接与rho mRNA的5′-UTR中的特定区域相互作用，保护该转录物免受其自身蛋白Rho的作用。转录终止是基因表达调控的关键步骤。主要终止机制之一是由Rho因子介导的，Rho因子在与RNA聚合酶结合时解离复杂的mRNA-DNA-RNA聚合酶。Rho促进操纵子末端的终止，但它也可以终止前导区内的转录，执行调节功能并避免普遍转录。rho的转录通过转录物的前导区中的Rho依赖性衰减来自动调节。在这项研究中，我们在这条途径中加入了一个额外的参与者。通过进行MS 2亲和纯化结合RNA测序（MAPS），rho转录本显示直接与小的非编码RNA SraL相互作用。使用生物信息学在体内和体外实验分析，SraL被证明与rho mRNA的5′-UTR碱基配对，在几种生长条件下上调其表达。这种碱基配对被证明可以阻止Rho对其自身信息的作用。此外，所获得的结果表明，ProQ和Hfq都与这种调节有关。我们提出了一个模型，考虑沙门氏菌SraL sRNA的作用，在保护rho mRNA从过早的转录终止Rho。请注意，由于两种RNA之间的相互作用区域对应于一个非常保守的序列，因此承认这种调节也发生在其他肠细菌中是合理的。
Significance Rho is an essential protein that promotes transcription termination at specific regions of the genome. Its activity is important not only at the end of genes, but also within leader regions where it has regulatory functions. This protein was shown to be involved in the regulation of rho mRNA promoting its premature transcription termination. In this study, we included an additional player in the complex pathway of regulation of this protein. Using in vivo and in vitro experiments, the small noncoding RNA SraL was shown to directly interact with a specific region in the 5′-UTR of rho mRNA protecting this transcript against the action of its own protein Rho. Transcription termination is a critical step in the control of gene expression. One of the major termination mechanisms is mediated by Rho factor that dissociates the complex mRNA-DNA-RNA polymerase upon binding with RNA polymerase. Rho promotes termination at the end of operons, but it can also terminate transcription within leader regions, performing regulatory functions and avoiding pervasive transcription. Transcription of rho is autoregulated through a Rho-dependent attenuation in the leader region of the transcript. In this study, we have included an additional player in this pathway. By performing MS2-affinity purification coupled with RNA sequencing (MAPS), rho transcript was shown to directly interact with the small noncoding RNA SraL. Using bioinformatic in vivo and in vitro experimental analyses, SraL was shown to base pair with the 5′-UTR of rho mRNA upregulating its expression in several growth conditions. This base pairing was shown to prevent the action of Rho over its own message. Moreover, the results obtained indicate that both ProQ and Hfq are associated with this regulation. We propose a model that contemplates the action of Salmonella SraL sRNA in the protection of rho mRNA from premature transcription termination by Rho. Note that since the interaction region between both RNAs corresponds to a very-well-conserved sequence, it is plausible to admit that this regulation also occurs in other enterobacteria.
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影响因子：
10.5
作者：
Wassarman, KM;Repoila, F;Gottesman, S
通讯作者：
Gottesman, S
影响因子：
30.3
作者：
Kroeger, Carsten;Colgan, Aoife;Hinton, Jay C. D.
通讯作者：
Hinton, Jay C. D.
影响因子：
5.4
作者：
Kavita, Kumari;de Mets, Francois;Gottesman, Susan
通讯作者：
Gottesman, Susan
影响因子：
7.2
作者：
Gottesman, Susan;Storz, Gisela
通讯作者：
Storz, Gisela
DOI：
10.1073/pnas.95.21.12462
发表时间：
1998-10-13
影响因子：
11.1
作者：
Majdalani, N;Cunning, C;Gottesman, S
通讯作者：
Gottesman, S