Safe and Efficacious Near Superhydrophobic Hemostat for Reduced Blood Loss and Easy Detachment in Traumatic Wounds.
Safe and Efficacious Near Superhydrophobic Hemostat for Reduced Blood Loss and Easy Detachment in Traumatic Wounds.
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DOI：
10.1021/acsami.3c12443
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发表时间：
2024-01-31
影响因子：
9.5
通讯作者：
Yap CH
中科院分区：
文献类型：
--
作者：
Dong Y;Xu Y;Lian C;Prak K;Leo HL;Tetley TD;Braga V;Emerson M;Ahnström J;Yap CH
出血是创伤死亡的主要原因，止血技术的创新至关重要。强疏水性碳纤维（CNF）涂层先前已被证明具有优异的止血性能。然而，CNF涂层如何引导凝血级联反应和CNF作为止血剂的生物安全性的理解还有待探索。在这里，我们的凝血酶生成试验研究表明，CNF通过因子（F）XII激活内源性途径诱导快速凝血。我们进一步对大鼠静脉损伤进行了研究，并证明与普通纱布和高岭土浸渍纱布（QuikClot）相比，CNF纱布能够大幅减少失血量。来自模型的血液样品的分析显示CNF纱布没有急性毒性，在任何器官中没有可检测到的CNF沉积，这表明CNF在我们的纱布上的固定防止了CNF渗透到血流中。还研究了将CNF直接注射到大鼠静脉中，发现不会引起明显的急性毒性或影响动物的存活或行为。最后，用原代角质形成细胞进行的毒性测定显示对CNF的毒性反应最小。因此，我们的研究支持了CNF止血纱布的安全性和有效性，突出了其作为止血控制领域有前景的方法的潜力。
Hemorrhage is the leading cause of trauma death, and innovation in hemostatic technology is important. The strongly hydrophobic carbon nanofiber (CNF) coating has previously been shown to have excellent hemostatic properties. However, the understanding of how CNF coating guides the coagulation cascade and the biosafety of CNF as hemostatic agents has yet to be explored. Here, our thrombin generation assay investigation showed that CNF induced fast blood coagulation via factor (F) XII activation of the intrinsic pathway. We further performed studies of a rat vein injury and demonstrated that the CNF gauze enabled a substantial reduction of blood loss compared to both the plain gauze and kaolin-imbued gauze (QuikClot). Analysis of blood samples from the model revealed no acute toxicity from the CNF gauze, with no detectable CNF deposition in any organ, suggesting that the immobilization of CNF on our gauze prevented the infiltration of CNF into the bloodstream. Direct injection of CNF into the rat vein was also investigated and found not to elicit overt acute toxicity or affect animal survival or behavior. Finally, toxicity assays with primary keratinocytes revealed minimal toxicity responses to CNF. Our studies thus supported the safety and efficacy of the CNF hemostatic gauze, highlighting its potential as a promising approach in the field of hemostatic control.
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影响因子：
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作者：
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通讯作者：
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