Comparison of cytotoxicity effects induced by four different types of nanoparticles in human corneal and conjunctival epithelial cells.
Comparison of cytotoxicity effects induced by four different types of nanoparticles in human corneal and conjunctival epithelial cells.
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DOI：
10.1038/s41598-021-04199-3
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发表时间：
2022-01-07
影响因子：
4.6
通讯作者：
Song JS
中科院分区：
文献类型：
--
作者：
Li X;Kang B;Eom Y;Zhong J;Lee HK;Kim HM;Song JS
近年来，颗粒物（PM）对眼表健康的影响引起了越来越多的关注。此前的研究表明，PM 化学成分的差异会影响毒理学反应。然而，关于 PM 化学成分对眼表毒性作用的现有信息不足。在本文中，我们旨在研究PM化学成分对眼表的毒性作用，重点研究四种不同类型的纳米颗粒（NP）对人角膜上皮细胞（HCEC）和人结膜上皮细胞（HCjEC）的影响，包括二氧化钛（TiO2）、炭黑（CB）、二氧化锌（ZnO）和二氧化硅（SiO2）。我们发现 CB、ZnO 和 SiO2 NP 的体外细胞毒性作用取决于颗粒特性和细胞类型以及暴露浓度和时间。这里，毒性增加的顺序是SiO2→CB→ZnO，而TiO2没有表现出毒性。此外，HCEC 的毒性作用比 HCjEC 更严重。活性氧（ROS）介导的氧化应激在HCEC和HCjEC中这三种NP的毒性中起着关键作用，导致细胞凋亡和线粒体损伤，这也是衰老的重要因素。 Sirtuin1 (SIRT1) 作为一种 NAD+ 依赖性蛋白脱乙酰酶，似乎在此过程中发挥潜在的保护作用。这些发现表明ROS和/或SIRT1可能成为临床治疗PM或NP相关眼表疾病的潜在靶点。
The impact of particulate matter (PM) on ocular surface health has attracted increased attention in recent years. Previous studies have reported that differences in the chemical composition of PM can affect the toxicological response. However, available information on the toxic effects of chemical components of PM on the ocular surface is insufficient. In this paper, we aimed to investigate the toxicity effects of chemical components of PM on the ocular surface, focusing on the effects of four different types of nanoparticles (NPs) in human corneal epithelial cells (HCECs) and human conjunctival epithelial cells (HCjECs), which include titanium dioxide (TiO2), carbon black (CB), zinc dioxide (ZnO), and silicon dioxide (SiO2). We found that the in vitro cytotoxic effects of CB, ZnO, and SiO2 NPs are dependent on particle properties and cell type as well as the exposure concentration and time. Here, the order of increasing toxicity was SiO2 → CB → ZnO, while TiO2 demonstrated no toxicity. Moreover, toxic effects appearing more severe in HCECs than HCjECs. Reactive oxygen species (ROS)-mediated oxidative stress plays a key role in the toxicity of these three NPs in HCECs and HCjECs, leading to apoptosis and mitochondrial damage, which are also important contributors to aging. Sirtuin1 (SIRT1) as an NAD+-dependent protein deacetylase that seems to play a potential protective role in this process. These findings implied that ROS and/or SIRT1 may become a potential target of clinical treatment of PM- or NP-related ocular surface diseases.
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影响因子：
64.5
作者：
López-Otín C;Blasco MA;Partridge L;Serrano M;Kroemer G
通讯作者：
Kroemer G
影响因子：
10.4
作者：
Long TC;Tajuba J;Sama P;Saleh N;Swartz C;Parker J;Hester S;Lowry GV;Veronesi B
通讯作者：
Veronesi B
影响因子：
4.4
作者：
Chang, Chia-Jen;Yang, Hsi-Hsien;Tsai, Hsien-Yang
通讯作者：
Tsai, Hsien-Yang
影响因子：
2.3
作者：
Hyun, Soo-Wang;Song, Su Jeong;Kim, Chan-Sik
通讯作者：
Kim, Chan-Sik
DOI：
10.1016/j.mrgentox.2019.01.009
发表时间：
2019-06-01
影响因子：
1.9
作者：
Cakmak, Gonca;Ari, Pelin Erturk;Gaga, Eftade O.
通讯作者：
Gaga, Eftade O.