Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis.
Functional and RNA-Sequencing Analysis Revealed Expression of a Novel Stay-Green Gene from Zoysia japonica (ZjSGR) Caused Chlorophyll Degradation and Accelerated Senescence in Arabidopsis.
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功能和 RNA 测序分析揭示了来自结缕草 (ZjSGR) 的新型保持绿色基因的表达导致拟南芥叶绿素降解和加速衰老
DOI：
10.3389/fpls.2016.01894
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发表时间：
2016
影响因子：
5.6
通讯作者：
Han L
中科院分区：
文献类型：
--
作者：
Teng K;Chang Z;Li X;Sun X;Liang X;Xu L;Chao Y;Han L
衰老是植物重要的发育过程，也是植物对生物和非生物胁迫的一种响应调节。保绿蛋白在植物衰老和叶绿素降解过程中起着重要作用。然而，潜在的机制没有得到很好的研究，特别是在非模式植物。本研究从结缕草中克隆了一个新的保绿基因ZjSGR。亚细胞定位结果表明ZjSGR定位于叶绿体中。实时荧光定量PCR和启动子活性测定结果表明，黑暗、阿坝和MeJA均可诱导ZjSGR的表达。它的表达水平也可以上调自然衰老，但抑制SA处理。ZjSGR在拟南芥中的过表达导致了快速变黄的表型;互补实验证明ZjSGR是拟南芥AtNYE 1的功能同源物。ZjSGR的过表达加速了拟南芥叶绿素的降解，并损害了光合作用。透射电子显微镜观察表明，ZjSGR的过表达破坏了叶绿体的结构。RNA测序分析表明，ZjSGR通过调节激素信号转导和大量衰老及环境胁迫相关基因的表达，在衰老和叶绿素降解中发挥多重作用。我们的研究提供了一个更好的理解SGRs的作用，并在植物衰老和叶绿素降解机制的新见解。
Senescence is not only an important developmental process, but also a responsive regulation to abiotic and biotic stress for plants. Stay-green protein plays crucial roles in plant senescence and chlorophyll degradation. However, the underlying mechanisms were not well-studied, particularly in non-model plants. In this study, a novel stay-green gene, ZjSGR, was isolated from Zoysia japonica. Subcellular localization result demonstrated that ZjSGR was localized in the chloroplasts. Quantitative real-time PCR results together with promoter activity determination using transgenic Arabidopsis confirmed that ZjSGR could be induced by darkness, ABA and MeJA. Its expression levels could also be up-regulated by natural senescence, but suppressed by SA treatments. Overexpression of ZjSGR in Arabidopsis resulted in a rapid yellowing phenotype; complementary experiments proved that ZjSGR was a functional homolog of AtNYE1 from Arabidopsis thaliana. Over expression of ZjSGR accelerated chlorophyll degradation and impaired photosynthesis in Arabidopsis. Transmission electron microscopy observation revealed that overexpression of ZjSGR decomposed the chloroplasts structure. RNA sequencing analysis showed that ZjSGR could play multiple roles in senescence and chlorophyll degradation by regulating hormone signal transduction and the expression of a large number of senescence and environmental stress related genes. Our study provides a better understanding of the roles of SGRs, and new insight into the senescence and chlorophyll degradation mechanisms in plants.
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影响因子：
4.8
作者：
Christ, Bastien;Hoertensteiner, Stefan
通讯作者：
Hoertensteiner, Stefan
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
12.3
作者：
Andersson A;Keskitalo J;Sjödin A;Bhalerao R;Sterky F;Wissel K;Tandre K;Aspeborg H;Moyle R;Ohmiya Y;Bhalerao R;Brunner A;Gustafsson P;Karlsson J;Lundeberg J;Nilsson O;Sandberg G;Strauss S;Sundberg B;Uhlen M;Jansson S;Nilsson P
通讯作者：
Nilsson P
影响因子：
4.2
作者：
DODGE, JD
通讯作者：
DODGE, JD
影响因子：
5.2
作者：
Guo, DP;Guo, YP;Rao, GZ
通讯作者：
Rao, GZ