Transient and persistent metabolomic changes in plasma following chronic cigarette smoke exposure in a mouse model.
Transient and persistent metabolomic changes in plasma following chronic cigarette smoke exposure in a mouse model.
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DOI：
10.1371/journal.pone.0101855
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Reisdorph N
中科院分区：
文献类型：
--
作者：
Cruickshank-Quinn CI;Mahaffey S;Justice MJ;Hughes G;Armstrong M;Bowler RP;Reisdorph R;Petrache I;Reisdorph N
接触香烟烟雾与易感人群罹患多种慢性肺部疾病和全身性疾病有关。代谢组学方法可能有助于定义疾病表型，可能有助于预测对治疗的反应，并且可以识别患病风险的生物标志物。使用慢性香烟烟雾暴露足以引起轻度肺气肿的小鼠模型，我们研究了香烟烟雾是否会诱导不同的代谢特征，并确定其在戒烟后的持续性。在进行基于液相色谱质谱的代谢组学之前，使用液-液和固相萃取从血浆中提取代谢物并根据化学类别进行分级。评估代谢物组平均值之间的统计显着性差异（p 值≤0.05）和倍数变化≥1.5）。与接触空气的动物相比，接触香烟烟雾的动物氨基酸、嘌呤、脂质、脂肪酸和类固醇代谢物水平存在显着差异。虽然 60% 的代谢物变化是可逆的，但 40% 的代谢物即使在戒烟 2 个月后仍然持续变化，包括尼古丁代谢物。对代谢物种类的验证以及将这些发现转化为吸烟引起的人类血浆代谢物特征可能会发现吸烟引起的疾病的生物标志物或致病途径。
Cigarette smoke exposure is linked to the development of a variety of chronic lung and systemic diseases in susceptible individuals. Metabolomics approaches may aid in defining disease phenotypes, may help predict responses to treatment, and could identify biomarkers of risk for developing disease. Using a mouse model of chronic cigarette smoke exposure sufficient to cause mild emphysema, we investigated whether cigarette smoke induces distinct metabolic profiles and determined their persistence following smoking cessation. Metabolites were extracted from plasma and fractionated based on chemical class using liquid-liquid and solid-phase extraction prior to performing liquid chromatography mass spectrometry-based metabolomics. Metabolites were evaluated for statistically significant differences among group means (p-value≤0.05) and fold change ≥1.5). Cigarette smoke exposure was associated with significant differences in amino acid, purine, lipid, fatty acid, and steroid metabolite levels compared to air exposed animals. Whereas 60% of the metabolite changes were reversible, 40% of metabolites remained persistently altered even following 2 months of smoking cessation, including nicotine metabolites. Validation of metabolite species and translation of these findings to human plasma metabolite signatures induced by cigarette smoking may lead to the discovery of biomarkers or pathogenic pathways of smoking-induced disease.
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影响因子：
4.5
作者：
通讯作者：
--
影响因子：
6
作者：
English, KM;Pugh, PJ;Jones, TH
通讯作者：
Jones, TH
DOI：
10.1164/rccm.200906-0826oc
发表时间：
2010-02-15
影响因子：
24.7
作者：
Diab, Khalil J.;Adamowicz, Jeremy J.;Petrache, Irina
通讯作者：
Petrache, Irina
影响因子：
9.6
作者：
Esther, Charles R., Jr.;Lazaar, Aili L.;Boucher, Richard C.
通讯作者：
Boucher, Richard C.
影响因子：
6.1
作者：
Aragon, M.;Marce, R. M.;Borrull, F.
通讯作者：
Borrull, F.