Cotinine and trans 3'-hydroxycotinine in dried blood spots as biomarkers of tobacco exposure and nicotine metabolism.
Cotinine and trans 3'-hydroxycotinine in dried blood spots as biomarkers of tobacco exposure and nicotine metabolism.
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DOI：
10.1038/jes.2013.7
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发表时间：
2013-09
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
在美国，吸烟是导致过早死亡的主要可预防原因。接触二手烟还会导致一些过早死亡以及其他负面健康后果。准确评估烟草烟雾暴露对了解这些疾病过程至关重要。可替宁是尼古丁的主要代谢物，它的血浆浓度被广泛接受为烟草和SHS暴露的定量测量。然而，收集血浆并不总是可行的。干血斑（DBS），通常从新生儿和幼儿收集铅筛查，提供了另一种抽样方法。建立了DBS中可替宁的高通量液相色谱串联质谱（LC/MS/MS）定量分析方法。定量限为0.3 ng/g （~ 0.2 ng/ml血浆）。测定了83名吸烟者和99名暴露于SHS的非吸烟者DBS中的可替宁水平。这些受试者的血浆可替宁浓度范围为<0.02至443 ng/ml。157例受试者的DBS中检出可替宁，血浆中可替宁与DBS的相关性为0.992 （p<0.001）。我们还测定了吸烟者DBS中反式-3′-羟基可替宁与可替宁的比值，这是尼古丁代谢的量度。DBS的这一比率与血浆的这一比率有良好的相关性，为0.94 （p<0.001）。在一项小型研究中，我们证实了使用现有DBS进行铅筛查以评估儿童SHS暴露的可行性。
Tobacco use is the major preventable cause of premature death in the United States. Secondhand smoke (SHS) exposure also contributes to a number of premature deaths as well as other negative health outcomes. An accurate assessment of tobacco smoke exposure is critical to understanding these disease processes. The plasma concentration of cotinine, the primary metabolite of nicotine, is widely accepted as a quantitative measure of tobacco and SHS exposure. However, it is not always feasible to collect plasma. Dried blood spots (DBS), which are collected routinely from newborns and often from young children for lead screening, provide an alternative sampling method. We have developed a quantitative high throughput liquid chromatography tandem mass spectrometry (LC/MS/MS) method for the analysis of cotinine in DBS. The limit of quantitation was 0.3 ng/g (~ 0.2 ng/ml plasma). Cotinine levels in DBS from 83 smokers and 99 nonsmokers exposed to SHS were determined. Plasma cotinine concentrations in these subjects ranged from <0.02 to 443 ng/ml. Cotinine was detected in DBS from 157 subjects, and the correlation between cotinine in plasma and DBS was excellent, 0.992 (p<0.001). We also determined the ratio of trans-3'-hydroxycotinine to cotinine, a measure of nicotine metabolism, in DBS from smokers. This ratio in DBS was well correlated with the ratio in plasma, 0.94 (p<0.001). In a small study we confirmed the feasibility of using extant DBS collected for lead screening to assess SHS exposure in children.
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尼古丁代谢物比预测吸烟地形和致癌生物标志物水平。
DOI：
10.1158/1055-9965.epi-10-0674
发表时间：
2011-02
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Strasser AA;Benowitz NL;Pinto AG;Tang KZ;Hecht SS;Carmella SG;Tyndale RF;Lerman CE
通讯作者：
Lerman CE
影响因子：
2.6
作者：
Bloom J;Hinrichs AL;Wang JC;von Weymarn LB;Kharasch ED;Bierut LJ;Goate A;Murphy SE
通讯作者：
Murphy SE
DOI：
10.1001/archpediatrics.2012.170
发表时间：
2012-09-01
影响因子：
--
作者：
Dempsey, Delia A.;Meyers, Matthew J.;Oh, Sam S.;Nguyen, Elizabeth A.;Fuentes-Afflick, Elena;Wu, Alan H. B.;Jacob, Peyton, III;Benowitz, Neal L.
通讯作者：
Benowitz, Neal L.
影响因子：
11.4
作者：
Spliethoff, Henry M.;Tao, Lin;Eadon, George A.
通讯作者：
Eadon, George A.
影响因子：
4.2
作者：
Mei, JV;Alexander, JR;Hannon, WH
通讯作者：
Hannon, WH