Silicon nanowires with high-k hafnium oxide dielectrics for sensitive detection of small nucleic acid oligomers.
Silicon nanowires with high-k hafnium oxide dielectrics for sensitive detection of small nucleic acid oligomers.
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DOI：
10.1021/nn301495k
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发表时间：
2012-07-24
期刊：
影响因子：
17.1
通讯作者：
Bashir, Rashid
中科院分区：
文献类型：
--
作者：
Dorvel, Brian R.;Reddy, Bobby, Jr.;Go, Jonghyun;Guevara, Carlos Duarte;Salm, Eric;Alam, Muhammad Ashraful;Bashir, Rashid
基于硅纳米线场效应晶体管的纳米生物传感器具有低成本、无标记检测和大规模并行化潜力的优点。因此，这些传感器经常被认为是床旁护理 (POC) 医疗诊断应用的有吸引力的选择。不幸的是，许多性能问题（例如由于流体接触而导致的栅极泄漏和电流不稳定）阻碍了该技术在日常使用中的广泛采用。高 k 电介质，例如氧化铪 (HfO2)，已知能够通过钝化暴露表面来应对离子传输不稳定的问题，从而应对这些挑战。随着这些基本稳定性问题的解决，POC 诊断和 SiNWFET 的一个有希望的目标是小寡核苷酸，更具体地说是 microRNA (miRNA)。 MicroRNA 是一种小 RNA 寡核苷酸，可与信使 RNA 结合，导致蛋白质翻译抑制、基因沉默，并且在多种癌症中表达通常会发生改变。在本文中，我们描述了一种用于生物传感应用的稳定 HfO2 电介质硅纳米线的制造工艺。在这里，我们展示了使用 miR-10b 和 miR-21 作为模板，对单链 DNA 类似物与其 microRNA 表亲进行感应，已知这两种物质在乳腺癌中都会上调。我们使用 miR-10b DNA 类似物作为靶序列和不同分子量的聚-L-赖氨酸作为功能化层来表征表面功能化对器件性能的影响。通过优化表面功能化和制造方案，我们能够实现 miR-10b DNA 类似物的 <100fM 检测水平，理论检测限为 1fM。此外，基于 miR-21 的非互补 DNA 靶链几乎没有反应，表明这是一个高度敏感和高度选择性的生物传感平台。
Nanobiosensors based on silicon nanowire field effect transistors offer advantages of low cost, label-free detection, and potential for massive parallelization. As a result, these sensors have often been suggested as an attractive option for applications in Point-of-care (POC) medical diagnostics. Unfortunately, a number of performance issues such as gate leakage and current instability due to fluid contact, have prevented widespread adoption of the technology for routine use. High-k dielectrics, such as hafnium oxide (HfO2), have the known ability to address these challenges by passivating the exposed surfaces against destabilizing concerns of ion transport. With these fundamental stability issues addressed, a promising target for POC diagnostics and SiNWFET’s has been small oligonucleotides, more specifically microRNA (miRNA). MicroRNA’s are small RNA oligonucleotides which bind to messenger RNA’s, causing translational repression of proteins, gene silencing, and expressions are typically altered in several forms of cancer. In this paper, we describe a process for fabricating stable HfO2 dielectric based silicon nanowires for biosensing applications. Here we demonstrate sensing of single stranded DNA analogues to their microRNA cousins using miR-10b and miR-21 as templates, both known to be upregulated in breast cancer. We characterize the effect of surface functionalization on device performance using the miR-10b DNA analogue as the target sequence and different molecular weight poly-l-lysine as the functionalization layer. By optimizing the surface functionalization and fabrication protocol, we were able to achieve <100fM detection levels of miR-10b DNA analogue, with a theoretical limit of detection of 1fM. Moreover, the non-complementary DNA target strand, based on miR-21, showed very little response, indicating a highly sensitive and highly selective biosensing platform.
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影响因子：
8.4
作者：
Bergveld, P
通讯作者：
Bergveld, P
影响因子：
3.2
作者：
Callegari, A;Cartier, E;Zabel, T
通讯作者：
Zabel, T
DOI：
10.2165/00129784-200101040-00003
发表时间：
2001-01-01
期刊：
American journal of cardiovascular drugs : drugs, devices, and other interventions
影响因子：
--
作者：
Douketis, J D
通讯作者：
Douketis, J D
影响因子：
4
作者：
Elibol, OH;Morisette, D;Bashir, R
通讯作者：
Bashir, R
影响因子：
--
作者：
Chen, Si;Nyholm, Leif;Zhang, Shi-Li
通讯作者：
Zhang, Shi-Li