Simultaneous profiling of 194 distinct receptor transcripts in human cells.
Simultaneous profiling of 194 distinct receptor transcripts in human cells.
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DOI：
10.1126/scisignal.2003624
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发表时间：
2013-08-06
影响因子：
7.3
通讯作者：
Janes KA
中科院分区：
文献类型：
--
作者：
Kang BH;Jensen KJ;Hatch JA;Janes KA
许多信号转导级联是由跨膜受体启动的，受体的存在或不存在以及丰度决定细胞的反应性。在这里，我们提供了一系列经过验证的定量逆转录聚合酶链反应 (qRT-PCR) 试剂，用于对人类基因组中 194 种跨膜受体的转录物的存在和相对丰度进行高通量分析。我们发现，与传统的寡核苷酸微阵列或外显子微阵列相比，qRT-PCR 阵列对检测的受体转录谱具有更高的灵敏度和特异性。 qRT-PCR 阵列还比腺苷酸化 RNA 种类的深度测序（RNA-seq）更准确地区分功能性受体的存在与否。通过对代表四种主要组织（胰腺、皮肤、乳腺和结肠）的 40 个人类细胞系应用基于 qRT-PCR 的受体转录谱分析，我们鉴定了具有增强信号传导能力的细胞系簇，并揭示了受体沉默在定义组织谱系中的作用。黑色素瘤细胞中白细胞介素 10 (IL-10) 受体编码基因 IL10RA 的异位表达参与了该细胞类型中不存在的 IL-10 自分泌环，从而改变了信号传导、基因表达和细胞对促炎刺激的反应。我们的阵列提供了一种快速、廉价且方便的方法，用于将受体特征分配给任何人类细胞或组织类型。
Many signal transduction cascades are initiated by transmembrane receptors with the presence or absence and abundance of receptors dictating cellular responsiveness. Here, we provide a validated array of quantitative reverse-transcription polymerase chain reaction (qRT-PCR) reagents for high-throughput profiling of the presence and relative abundance of transcripts for 194 transmembrane receptors in the human genome. We found that the qRT-PCR array had greater sensitivity and specificity for the detected receptor transcript profiles compared to conventional oligonucleotide microarrays or exon microarrays. The qRT-PCR array also distinguished functional receptor presence versus absence more accurately than deep sequencing of adenylated RNA species, RNA-seq. By applying qRT-PCR-based receptor transcript profiling to 40 human cell lines representing four main tissues (pancreas, skin, breast, and colon), we identified clusters of cell lines with enhanced signaling capabilities and revealed a role for receptor silencing in defining tissue lineage. Ectopic expression of the interleukin 10 (IL-10) receptor encoding gene IL10RA in melanoma cells engaged an IL-10 autocrine loop not otherwise present in this cell type, which altered signaling, gene expression, and cellular responses to proinflammatory stimuli. Our array provides a rapid, inexpensive, and convenient means for assigning a receptor signature to any human cell or tissue type.
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影响因子：
4.8
作者：
Debnath, J;Muthuswamy, SK;Brugge, JS
通讯作者：
Brugge, JS
影响因子：
--
作者：
Ben-Shlomo, Izhar;Rauch, Rami;Hsueh, Aaron J. W.
通讯作者：
Hsueh, Aaron J. W.
影响因子：
16
作者：
Bruning, JC;Michael, MD;Kahn, CR
通讯作者：
Kahn, CR
DOI：
10.1126/stke.2003.187.re9
发表时间：
2003-06-17
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
--
作者：
Ben-Shlomo, Izhar;Yu Hsu, Sheau;Hsueh, Aaron J W
通讯作者：
Hsueh, Aaron J W
DOI：
10.1073/pnas.74.2.565
发表时间：
1977-01-01
影响因子：
11.1
作者：
FABRICANT, RN;DELARCO, JE;TODARO, GJ
通讯作者：
TODARO, GJ