PiT2 regulates neuronal outgrowth through interaction with microtubule-associated protein 1B.
PiT2 regulates neuronal outgrowth through interaction with microtubule-associated protein 1B.
复制标题
PiT2 通过与微管相关蛋白 1B 相互作用调节神经元生长
DOI：
10.1038/s41598-017-17953-3
复制
发表时间：
2017-12-19
影响因子：
4.6
通讯作者：
Liu JY
中科院分区：
文献类型：
--
作者：
Ma XX;Li X;Yi P;Wang C;Weng J;Zhang L;Xu X;Sun H;Feng S;Liu K;Chen R;Du S;Mao X;Zeng X;Zhang LY;Liu M;Tang BS;Zhu X;Jin S;Liu JY
PiT2是无机磷转运蛋白家族的成员，在神经系统中广泛表达。研究发现，PiT2的loop7结构域不是逆转录病毒识别和转运功能所必需的。目前对loop7的确切功能仍知之甚少。在这里，我们证明了PiT2的环7对于PiT2蛋白到细胞表面的运输是必要的。此外，loop7还与神经2A细胞中突起的生长有关，它与微管相关蛋白1B(MAP1B)的轻链1相互作用。MAP1B结合位点突变的PiT2影响轴突生长，而PI转运功能缺失的PiT2突变体则不影响轴突生长。我们还发现，果蝇PiT与微管相关蛋白Futsch相互作用，dPiT对神经肌肉接头(NMJ)的正常发育至关重要。这些结果表明，PiT2可能通过与MAP1B相互作用而不依赖于其在神经系统中的PI转运功能来参与神经元生长的调节。
PiT2 is a member of the inorganic phosphate transporter family, and is extensively expressed in the nervous system. It was found that loop7 domain of PiT2 is not required for retroviral recognition and transport function. The exact functions of loop7 remain poorly understood. Here we show that loop7 of PiT2 is necessary for the transport of PiT2 protein to the cell surface. Further, loop7 is also related to the outgrowth of neurite in Neuro2A cells interacts with the light chain 1 of microtubule-associated protein 1B (MAP1B). PiT2 with mutated MAP1B binding sites affect neurite outgrowth whereas Pi transport function deficient mutants of PiT2 do not. We also show thatDrosophiladPiT interacts with microtubule-associated protein Futsch, and dPiT is crucial for the normal development of neuromuscular junctions (NMJs). These results indicate that PiT2 might participate in the regulation of neuronal outgrowth by interacting with MAP1B and independently of its Pi transport function in the nervous system.
登录
查看更多内容
影响因子：
11
作者：
Lagrue E;Abe H;Lavanya M;Touhami J;Bodard S;Chalon S;Battini JL;Sitbon M;Castelnau P
通讯作者：
Castelnau P
影响因子：
5.3
作者：
Bouquet, C;Soares, S;Nothias, F
通讯作者：
Nothias, F
DOI：
10.1073/pnas.91.1.78
发表时间：
1994-01-04
影响因子：
11.1
作者：
MILLER, DG;EDWARDS, RH;MILLER, AD
通讯作者：
MILLER, AD
影响因子：
2.1
作者：
Nishimura, Masuhiro;Naito, Shinsaku
通讯作者：
Naito, Shinsaku
影响因子：
2.2
作者：
Hsu, Sandy Chan;Sears, Renee L.;Lemos, Roberta R.;Quintans, Beatriz;Huang, Alden;Spiteri, Elizabeth;Nevarez, Lisette;Mamah, Catherine;Zatz, Mayana;Pierce, Kerrie D.;Fullerton, Janice M.;Adair, John C.;Berner, Jon E.;Bower, Matthew;Brodaty, Henry;Carmona, Olga;Dobricic, Valerija;Fogel, Brent L.;Garcia-Estevez, Daniel;Goldman, Jill;Goudreau, John L.;Hopfer, Suellen;Jankovic, Milena;Jauma, Serge;Jen, Joanna C.;Kirdlarp, Suppachok;Klepper, Joerg;Kostic, Vladimir;Lang, Anthony E.;Linglart, Agnes;Maisenbacher, Melissa K.;Manyam, Bala V.;Mazzoni, Pietro;Miedzybrodzka, Zofia;Mitarnun, Witoon;Mitchell, Philip B.;Mueller, Jennifer;Novakovic, Ivana;Paucar, Martin;Paulson, Henry;Simpson, Sheila A.;Svenningsson, Per;Tuite, Paul;Vitek, Jerrold;Wetchaphanphesat, Suppachok;Williams, Charles;Yang, Michele;Schofield, Peter R.;de Oliveira, Joao R. M.;Sobrido, Maria-Jesus;Geschwind, Daniel H.;Coppola, Giovanni
通讯作者：
Coppola, Giovanni