Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport.
Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport.
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DOI：
10.1186/1423-0127-17-91
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发表时间：
2010-12-04
影响因子：
11
通讯作者：
Castelnau P
中科院分区：
文献类型：
--
作者：
Lagrue E;Abe H;Lavanya M;Touhami J;Bodard S;Chalon S;Battini JL;Sitbon M;Castelnau P
类人猿白血病病毒（GALV）、嗜中性鼠白血病病毒（AMLV）和人T细胞白血病病毒（HTLV）是在进入宿主细胞时特异性结合营养转运蛋白及其包膜糖蛋白（Env）的逆转录病毒。在这里，我们使用来自GALV，AMLV和HTLV Env的标记配体来监测其同源受体的分布，无机磷酸盐转运蛋白PiT 1和PiT 2，以及葡萄糖转运蛋白GLUT 1，分别在基础条件下和急性能量缺乏后。为了这个目的，我们监测了1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP），一种线粒体复合物I抑制剂，诱导纹状体-黑质网络神经元变性后，C57/BL 6小鼠小脑，额叶皮质，胼胝体，纹状体和黑质（SN）中PiT 1，PiT 2和GLUT 1分布的变化。PiT 1配体染色胼胝体中的少突胶质细胞，并在SN中显示网状图案。PiT 2配体染色特别是小脑浦肯野细胞，而GLUT 1标记主要观察到整个皮质，基底节和小脑灰质。有趣的是，不像GLUT 1和PiT 2分布，似乎没有被MPTP中毒修改，PiT 1免疫染色似乎在SN中更广泛。这种变化的合理原因，急性能源压力进行了讨论。因此，这些新的配体构成了新的代谢标志物，这将有助于解开细胞适应各种各样的正常和病理条件，并确定特定的营养转运蛋白在组织稳态中的作用。
The gibbon ape leukemia virus (GALV), the amphotropic murine leukemia virus (AMLV) and the human T-cell leukemia virus (HTLV) are retroviruses that specifically bind nutrient transporters with their envelope glycoproteins (Env) when entering host cells. Here, we used tagged ligands derived from GALV, AMLV, and HTLV Env to monitor the distribution of their cognate receptors, the inorganic phosphate transporters PiT1 and PiT2, and the glucose transporter GLUT1, respectively, in basal conditions and after acute energy deficiency. For this purpose, we monitored changes in the distribution of PiT1, PiT2 and GLUT1 in the cerebellum, the frontal cortex, the corpus callosum, the striatum and the substantia nigra (SN) of C57/BL6 mice after administration of 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridinium (MPTP), a mitochondrial complex I inhibitor which induces neuronal degeneration in the striato-nigral network. The PiT1 ligand stained oligodendrocytes in the corpus callosum and showed a reticular pattern in the SN. The PiT2 ligand stained particularly the cerebellar Purkinje cells, while GLUT1 labelling was mainly observed throughout the cortex, basal ganglia and cerebellar gray matter. Interestingly, unlike GLUT1 and PiT2 distributions which did not appear to be modified by MPTP intoxication, PiT1 immunostaining seemed to be more extended in the SN. The plausible reasons for this change following acute energy stress are discussed. These new ligands therefore constitute new metabolic markers which should help to unravel cellular adaptations to a wide variety of normal and pathologic conditions and to determine the role of specific nutrient transporters in tissue homeostasis.
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影响因子：
14.8
作者：
Jackson-Lewis, Vernice;Przedborski, Serge
通讯作者：
Przedborski, Serge
影响因子：
3.3
作者：
Choeiri, C;Staines, W;Messier, C
通讯作者：
Messier, C
影响因子：
2.9
作者：
Lee, Joo-Yong;Son, Hyo Jin;Koh, Jae-Young
通讯作者：
Koh, Jae-Young
影响因子：
11.2
作者：
Hartig, MB;Hörtnagel, K;Meitinger, T
通讯作者：
Meitinger, T
影响因子：
20.3
作者：
Montel-Hagen, Amelie;Blanc, Lionel;Taylor, Naomi
通讯作者：
Taylor, Naomi