Single-neuronal cell culture and monitoring platform using a fully transparent microfluidic DEP device.
Single-neuronal cell culture and monitoring platform using a fully transparent microfluidic DEP device.
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DOI：
10.1038/s41598-018-31576-2
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发表时间：
2018-09-04
影响因子：
4.6
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Kim H;Lee IK;Taylor K;Richters K;Baek DH;Ryu JH;Cho SJ;Jung YH;Park DW;Novello J;Bong J;Suminski AJ;Dingle AM;Blick RH;Williams JC;Dent EW;Ma Z
使用多电极阵列的介电泳可以与生物细胞建立非侵入性接口，用于长期监测电生理参数，以及用于神经元细胞操作的无标记和非破坏性技术。然而，利用介电泳进行神经元细胞操作的实验受到了限制，因为在细胞操作过程中，介电电泳装置通常在受控环境（即培养箱）之外运行，这是有问题的，因为神经元对理化环境的特性非常敏感。此外，设计用于产生介电泳力的传统多电极阵列通常是用不透明材料制造的，这会干扰活细胞成像。在这里，我们提出了一种先进的单神经元细胞培养和监测平台，使用完全透明的微流体介电电泳装置来不间断地监测神经元细胞的发育和功能。该设备安装在密封的孵化室内，以确保改善体内平衡条件并降低污染风险。因此，我们成功地在所需位置捕获和培养单个神经元，并在一周内监测它们的生长过程。所提出的单神经元细胞培养和监测平台不仅具有实现体外有序神经元网络的巨大潜力，而且还为神经元网络的广泛神经学研究和电生理学研究提供了有用的工具。
Dielectrophoresis using multi-electrode arrays allows a non-invasive interface with biological cells for long-term monitoring of electrophysiological parameters as well as a label-free and non-destructive technique for neuronal cell manipulation. However, experiments for neuronal cell manipulation utilizing dielectrophoresis have been constrained because dielectrophoresis devices generally function outside of the controlled environment (i.e. incubator) during the cell manipulation process, which is problematic because neurons are highly susceptible to the properties of the physiochemical environment. Furthermore, the conventional multi-electrode arrays designed to generate dielectrophoretic force are often fabricated with non-transparent materials that confound live-cell imaging. Here we present an advanced single-neuronal cell culture and monitoring platform using a fully transparent microfluidic dielectrophoresis device for the unabated monitoring of neuronal cell development and function. The device is mounted inside a sealed incubation chamber to ensure improved homeostatic conditions and reduced contamination risk. Consequently, we successfully trap and culture single neurons on a desired location and monitor their growth process over a week. The proposed single-neuronal cell culture and monitoring platform not only has significant potential to realize an in vitro ordered neuronal network, but also offers a useful tool for a wide range of neurological research and electrophysiological studies of neuronal networks.
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影响因子：
5.6
作者：
Gross A;Schoendube J;Zimmermann S;Steeb M;Zengerle R;Koltay P
通讯作者：
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Dent, Erik W.
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通讯作者：
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