Hybrid dysgenesis in Drosophila simulans lines transformed with autonomous P elements.
Hybrid dysgenesis in Drosophila simulans lines transformed with autonomous P elements.
复制标题
用自主 P 元件转化的模拟果蝇品系中的杂交发育不全。
作者：
Stephen B. Daniels;A. Chovnick;M. G. Kidwell
几个先前描述的P元件转化线的果蝇simulans的分子和表型分析延长，以确定它们是否有可能产生一个综合征的P-M杂交dysgenesis类似的果蝇。在分析开始时具有最高P元素数量的转化系DsPpi-5C具有强的P活性潜力和P元素调节，具有D. melanogaster P菌株。随后的分析来自34个单对交配的DsP pi-5C的亚系显示，他们是异质性的，就他们的P元件的补充和P活性的潜力，但类似的，就他们的监管能力。磷酶活性最高的亚系DsPpi-5C-27作为参比菌株，用于D. simulans转化子在这些试验中，观察到了与性腺不育和雄性重组有关的互交效应。就像D。melanogaster，诱导D.模拟显示出温度依赖性。DsP pi-5C-27的分子分析显示，它有大约30个P元件每个基因组，其中至少有一些是有缺陷的。其基因组中潜在完整P元件的数量与D.黑腹菌P株，Harwich-77。总体上，我们的分析表明，D. simulans能够表达先前在D. melanogaster和P元素似乎在两个兄弟物种中以类似的方式表现。
The molecular and phenotypic analysis of several previously described P element-transformed lines of Drosophila simulans was extended in order to determine whether they had the potential to produce a syndrome of P-M hybrid dysgenesis analogous to the one in Drosophila melanogaster. The transformed line with the highest number of P elements at the beginning of the analysis, DsP pi-5C, developed strong P activity potential and P element regulation, properties characteristic of D. melanogaster P strains. The subsequent analysis of sublines derived from 34 single pair matings of DsP pi-5C revealed that they were heterogeneous with respect to both their P element complements and P activity potentials, but similar with respect to their regulatory capabilities. The subline with the highest P activity, DsP pi-5C-27, was subsequently used as a reference P strain in the genetic analysis of the D. simulans transformants. In these experiments, the reciprocal cross effect was observed with respect to both gonadal sterility and male recombination. As in D. melanogaster, the induction of gonadal sterility in D. simulans was shown to be temperature-dependent. Molecular analysis of DsP pi-5C-27 revealed that it has approximately 30 P elements per genome, at least some of which are defective. The number of potentially complete P elements in its genome is similar to the number in the D. melanogaster P strain, Harwich-77. Overall our analysis indicates that P-transformed lines of D. simulans are capable of expressing the major features of P-M hybrid dysgenesis previously demonstrated in D. melanogaster and that P elements appear to behave in a similar way in the two sibling species.
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果蝇种间 DNA 转化。
DOI：
10.1073/pnas.81.23.7515
发表时间：
1984
影响因子：
11.1
作者：
Scavarda,NJ;Hartl,DL
通讯作者：
Hartl,DL
具有自主 P 元件的果蝇遗传转化：长期建立的转化品系的表型和分子分析。
影响因子：
3.3
作者：
Daniels,SB;Clark,SH;Kidwell,MG;Chovnick,A
通讯作者：
Chovnick,A
果蝇玫瑰色位点异色位置效应机制研究
影响因子：
3.3
作者：
Rushlow,CA;Bender,W;Chovnick,A
通讯作者：
Chovnick,A
哺乳动物细胞和酵母中果蝇 P 元件转座酶活性的证据。
影响因子：
5.6
作者：
Rio,DC;Barnes,G;Laski,FA;Rine,J;Rubin,GM
通讯作者：
Rubin,GM
不同种群的分子特征与最近移动 P 元素入侵黑腹果蝇的假设一致。
影响因子：
10.7
作者：
Anxolabéhère,D;Kidwell,MG;Periquet,G
通讯作者：
Periquet,G