Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage
Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage
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CpG ODN 激活巨噬细胞中差异表达 lncRNA 的鉴定
DOI：
10.1155/2020/1407654
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发表时间：
2020-06
影响因子：
4.1
通讯作者：
Zhou Zhiguang
中科院分区：
文献类型：
--
作者：
Zheng Ying;Luo Xi;Qin Zailong;Zhou Zhiguang
巨噬细胞是先天免疫的重要组成部分，其可被感染激活。产生和释放一系列炎性细胞因子以消除病原体。CpG DNA是由TLR 9识别的免疫刺激物，随后在巨噬细胞中诱导炎症反应。长链非编码RNA（longnoncodingRNA，lncRNA）是一类长度大于200 nt，但不编码蛋白质的新型非编码RNA。lncRNA参与许多生理和病理过程，包括炎症反应。在我们的研究中，lncRNA微阵列分析，以确定差异表达的lncRNA和mRNA在RAW264.7细胞在不同时间点后，CpG ODN刺激。结果表明，734 lncRNA和734 mRNA的表达水平在所有时间点都发生了变化。基因本体论（GO）和京都基因和基因组百科全书（KEGG）的生物学途径分析进行预测失调基因的功能。基于差异表达的lncRNA与10种已报道参与CpG DNA诱导的炎症反应的上调mRNA之间的相关性分析，构建lncRNA-mRNA的共表达网络。此外，我们选择了8个失调的lncRNA，通过定量实时PCR进行进一步验证。本研究对lncRNA在CpG ODN诱导的巨噬细胞活化中的潜在功能提供了系统的观点。
A macrophage is an important component of innate immunity which can be activated by infection. A series of inflammatory cytokines are produced and released to eliminate pathogens. CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages. Long noncoding RNA (lncRNA) is a novel class of noncoding RNA, whose length is more than 200 nt, but without protein-coding capacity. lncRNAs are involved in many physiological and pathological processes, including inflammatory responses. In our study, a lncRNA microarray assay was performed to identify differentially expressed lncRNAs and mRNAs in RAW264.7 cells at different time points following CpG ODN stimulation. The results revealed that expression levels of 734 lncRNAs and 734 mRNAs were altered at all time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed to predict the functions of dysregulated genes. Coexpression networks of lncRNA-mRNA were constructed based on the correlation analysis between differentially expressed lncRNAs and 10 selected upregulated mRNAs, which have been reported to be involved in CpG DNA-induced inflammatory responses. In addition, we selected 8 dysregulated lncRNAs for further validation by quantitative real-time PCR. The present study provided a systematic perspective on the potential functions of lncRNAs in CpG ODN-induced macrophage activation.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
4.6
作者：
Utaisincharoen, P;Anuntagool, N;Sirisinha, S
通讯作者：
Sirisinha, S
lncRNA 调节先天免疫反应及其在巨噬细胞极化中的作用
影响因子：
4.6
作者：
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通讯作者：
Zheng Y
影响因子：
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作者：
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通讯作者：
Huang, S. Joseph
破骨细胞生成过程中 lncRNA、mRNA、circRNA 和 miRNA 表达谱的变化。
影响因子：
4.6
作者：
Dou C;Cao Z;Yang B;Ding N;Hou T;Luo F;Kang F;Li J;Yang X;Jiang H;Xiang J;Quan H;Xu J;Dong S
通讯作者：
Dong S