Three-dimensional 10% cyclic strain reduces bovine aortic endothelial cell angiogenic sprout length and augments tubulogenesis in tubular fibrin hydrogels.
Three-dimensional 10% cyclic strain reduces bovine aortic endothelial cell angiogenic sprout length and augments tubulogenesis in tubular fibrin hydrogels.
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DOI：
10.1002/term.323
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发表时间：
2011-05
影响因子：
3.3
通讯作者：
Greisler, Howard P.
中科院分区：
文献类型：
--
作者：
Gassman, Andrew A.;Kuprys, Tomas;Ucuzian, Areck A.;Brey, Eric;Matsumura, Akie;Pang, Yonggang;Larsons, Jef;Greisler, Howard P.
功能性微脉管系统的发展对于植入的组织工程结构的长期存活至关重要。动态培养条件已证明可以显着调节表型特征并刺激基于水凝胶的组织工程血管内的细胞增殖。尽管先前的工作已经描述了单轴或等双轴机械刺激对内皮细胞的影响，但没有工作概述三维机械刺激对管状血管类似物内的内皮细胞的影响。我们在此证明，7 天的 10% 循环体积扩张对源自牛主动脉内皮细胞颗粒的血管生成芽的平均长度和密度具有有害影响。尽管两组都表现出内腔形成，但在动态培养条件下生长的芽通常具有更宽、分枝更少的芽模式。这些结果表明，长时间的机械刺激可能代表血管生成芽优先发展更大的管腔而不是细胞迁移和随后的芽长度的提示。
The development of a functional microvasculature is critical to the long-term survival of implanted tissue-engineered constructs. Dynamic culture conditions have shown to significantly modulate phenotypic characteristics and stimulate proliferation of cells within hydrogel-based tissue engineered blood vessels. Although prior work has described the effects uniaxial or equibiaxial mechanical stimulation has on endothelial cells, no work has outlined effects of three-dimensional mechanical stimulation on endothelial cells within tubular vessel analogs. We demonstrate here that 7 days of 10% cyclic volumetric distension has a deleterious effect on the average length and density of angiogenic sprouts derived from pellets of bovine aortic endothelial cells. Although both groups demonstrated lumen formation, the sprouts grown under dynamic culture conditions typically had wider, less-branching sprout patterns. These results suggest that prolonged mechanical stimulation could represent a cue for angiogenic sprouts to preferentially develop larger lumens over cellular migration and subsequent sprout length.
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影响因子：
4.8
作者：
Korff, T;Kimmina, S;Augustin, HG
通讯作者：
Augustin, HG
DOI：
10.1007/bf02616210
发表时间：
1978-01-01
期刊：
IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION
影响因子：
--
作者：
SCHWARTZ, SM
通讯作者：
SCHWARTZ, SM
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
--
作者：
Griffith, CK;Miller, C;George, SC
通讯作者：
George, SC
影响因子：
4.3
作者：
SUMPIO, BE;BANES, AJ;JOHNSON, G
通讯作者：
JOHNSON, G