Characterization and expression profiling of ATP-binding cassette transporter genes in the diamondback moth, Plutella xylostella (L.).
Characterization and expression profiling of ATP-binding cassette transporter genes in the diamondback moth, Plutella xylostella (L.).
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小菜蛾 (Plutella xylostella (L.)) 中 ATP 结合盒转运蛋白基因的特征和表达谱
DOI：
10.1186/s12864-016-3096-1
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发表时间：
2016-09-27
期刊：
影响因子：
4.4
通讯作者：
You M
中科院分区：
文献类型：
--
作者：
Qi W;Ma X;He W;Chen W;Zou M;Gurr GM;Vasseur L;You M
背景：ATP结合盒（ABC）转运蛋白是所有生物体中发现的主要跨膜蛋白家族之一，在多种化合物跨细胞内外膜转运中发挥重要作用。在某些物种中，ABC转运蛋白可能参与杀虫剂等物质的解毒。小菜蛾Plutella xylostella（L.）结果：从已发表的小菜蛾基因组中鉴定出82个ABC基因，根据系统发育分析将其分为8个亚科（ABCA-H）。与Bombyx mori、Manduca sexta、Heliconius melpomene、Danaus plexippus、Drosophila melanogaster、Tetranychus urtae和Homo sapiens的基因相比，发现小菜蛾中ABCA、ABCC和ABCH亚科的基因均发生了扩增。系统发育分析表明，小菜蛾中的许多ABC转运蛋白基因与其他7个物种中已被充分研究的ABC转运蛋白基因是同源的。基于转录组和qRT-PCR的分析阐明了小菜蛾ABC基因表达的生理效应，其具有发育阶段特异性和组织特异性，以及受昆虫是否来自抗性品系的影响。两个ABCC和一个ABCA基因在敏感品系（福州-S）的4龄幼虫中肠中优先表达，表明它们可能在植物防御物质的代谢中起作用。结论：本研究是迄今为止对小菜蛾ABC转运蛋白基因的鉴定、鉴定和表达谱分析最全面的研究。该基因家族的多样性特征和表达模式可能与该物种对广泛的杀虫剂和生物毒素产生抗性的进化能力有关。本研究结果为进一步研究小菜蛾特异性ABC转运蛋白基因的功能，以及进一步了解其在杀虫剂抗性中的生理作用和调控途径奠定了基础。
Background:ATP-binding cassette (ABC) transporters are one of the major transmembrane protein families found in all organisms and play important roles in transporting a variety of compounds across intra and extra cellular membranes. In some species, ABC transporters may be involved in the detoxification of substances such as insecticides. The diamondback moth, Plutella xylostella (L.), a destructive pest of cruciferous crops worldwide, is an important species to study as it is resistant to many types of insecticides as well as biological control Bacillus thuringiensis toxins.Results:A total of 82 ABC genes were identified from our published P. xylostella genome, and grouped into eight subfamilies (ABCA-H) based on phylogenetic analysis. Genes of subfamilies ABCA, ABCC and ABCH were found to be expanded in P. xylostella compared with those in Bombyx mori, Manduca sexta, Heliconius melpomene, Danaus plexippus, Drosophila melanogaster, Tetranychus urticae and Homo sapiens. Phylogenetic analysis indicated that many of the ABC transporters in P. xylostella are orthologous to the well-studied ABC transporter genes in the seven other species. Transcriptome- and qRT-PCR-based analysis elucidated physiological effects of ABC gene expressions of P. xylostella which were developmental stage- and tissue-specific as well as being affected by whether or not the insects were from an insecticide-resistant strain. Two ABCC and one ABCA genes were preferentially expressed in midgut of the 4th-instar larvae of a susceptible strain (Fuzhou-S) suggesting their potential roles in metabolizing plant defensive chemicals. Most of the highly expressed genes in insecticide-resistant strains were also predominantly expressed in the tissues of Malpighian tubules and midgut.Conclusions:This is the most comprehensive study on identification, characterization and expression profiling of ABC transporter genes in P. xylostella to date. The diversified features and expression patterns of this gene family may be associated with the evolutionary capacity of this species to develop resistance to a wide range of insecticides and biological toxins. Our findings provide a solid foundation for future functional studies on specific ABC transporter genes in P. xylostella, and for further understanding of their physiological roles and regulatory pathways in insecticide resistance.
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