Protein adsorption and transport in polymer-functionalized ion-exchangers.
Protein adsorption and transport in polymer-functionalized ion-exchangers.
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DOI：
10.1016/j.chroma.2011.06.061
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发表时间：
2011-12-09
期刊：
影响因子：
--
通讯作者：
Lenhoff AM
中科院分区：
文献类型：
--
作者：
Lenhoff AM
各种各样的固定相可用于蛋白质的制备色谱，涵盖不同的基础基质，孔结构和色谱模式。最近，这种材料的数量显著增加，其中基础基质被衍生化以添加共价连接或接枝的聚合物层，或者在某些情况下，添加填充孔隙空间的水凝胶。这篇综述总结了这类离子交换剂的主要结构和功能特征，这是此类材料中最大的一类。虽然吸附和传输特性通常可以使用与用于常规介质中的蛋白质相同的方法在操作上和现象学上建模，但这些吸附剂中的蛋白质行为存在值得注意的机制差异。蛋白质保留的一个根本区别是，它可以被描绘为分配到一个三维的聚合物相，而不是在一个扩展的二维表面吸附，如适用于更传统的媒体。然而，除了这种分配行为之外，聚合物官能化的介质通常显示快速的颗粒内传输，其虽然在定性上与常规介质中的颗粒内传输相当，但在某些条件下在定量上足够快速，其可以在性能的关键测量（例如动态结合能力）中产生明显的益处。虽然可能的保留和传输性能的机械基础进行了讨论，明显的不确定性领域，使详细的机械建模非常具有挑战性，更详细的实验表征可能是更富有成效的。
A wide variety of stationary phases is available for use in preparative chromatography of proteins, covering different base matrices, pore structures and modes of chromatography. There has recently been significant growth in the number of such materials in which the base matrix is derivatized to add a covalently attached or grafted polymer layer or, in some cases, a hydrogel that fills the pore space. This review summarizes the main structural and functional features of ion exchangers of this kind, which represent the largest class of such materials. Although the adsorption and transport properties may generally be used operationally and modeled phenomenologically using the same methods as are used for proteins in conventional media, there are noteworthy mechanistic differences in protein behavior in these adsorbents. A fundamental difference in protein retention is that it may be portrayed as partitioning into a three-dimensional polymer phase rather than adsorption at an extended two-dimensional surface, as applies in more conventional media. Beyond this partitioning behavior, however, the polymer-functionalized media often display rapid intraparticle transport that, while qualitatively comparable to that in conventional media, is sufficiently rapid quantitatively under certain conditions that it can lead to clear benefits in key measures of performance such as the dynamic binding capacity. Although possible mechanistic bases for the retention and transport properties are discussed, appreciable areas of uncertainty make detailed mechanistic modeling very challenging, and more detailed experimental characterization is likely to be more productive.
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影响因子：
4.1
作者：
Frankovic, Vida;Podgornik, Ales;Strancar, Ales
通讯作者：
Strancar, Ales
DOI：
10.1016/j.chroma.2009.09.014
发表时间：
2009-11-06
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Bowes BD;Koku H;Czymmek KJ;Lenhoff AM
通讯作者：
Lenhoff AM
DOI：
10.1073/pnas.0237084100
发表时间：
2003-01-21
影响因子：
11.1
作者：
Dziennik, SR;Belcher, EB;Lenhoff, AM
通讯作者：
Lenhoff, AM
影响因子：
4.1
作者：
Chang, C;Lenhoff, AM
通讯作者：
Lenhoff, AM
影响因子：
4.1
作者：
BOARDMAN, NK;PARTRIDGE, SM
通讯作者：
PARTRIDGE, SM