Standing genetic variation in contingency loci drives the rapid adaptation of Campylobacter jejuni to a novel host.
Standing genetic variation in contingency loci drives the rapid adaptation of Campylobacter jejuni to a novel host.
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应急基因座中的站立遗传变异驱动了弯曲杆菌的空肠对新宿主的快速适应。
DOI：
10.1371/journal.pone.0016399
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发表时间：
2011-01-24
期刊：
影响因子：
3.7
通讯作者：
Mansfield LS
中科院分区：
文献类型：
--
作者：
Jerome JP;Bell JA;Plovanich-Jones AE;Barrick JE;Brown CT;Mansfield LS
食源性病原体空肠弯曲菌的基因组包含多个高度可变的位点，或应急位点。有人认为，这些基因座的常设变异是快速适应新环境的一种机制，但这种现象尚未在实验中得到证实。在前期的工作中，我们发现C.空肠NCTC 11168在通过弯曲杆菌病的C57 BL/6 IL-10-/-小鼠模型连续传代后增加。在这里，我们试图确定这种适应过程中的遗传基础。将1.64Mb基因组重新测序至200- 500 X覆盖度，使我们能够在体内适应前后将23个偶然位点的变异定义到前所未有的深度。小鼠适应的C.空肠在很大程度上限于13个应变位点的同源聚合物束。这些变化导致表面结构生物合成基因座和仅具有推定功能的基因的开放阅读框的显著改变。几个具有开放阅读框变化的基因座也具有改变的转录本丰度。在体内传代过程中，C. jejuni，再加上观察到的毒力增加和缺乏其他类型的遗传变化，是第一个实验证据表明，这些可变区在C.空肠适应和新宿主中的毒力。
The genome of the food-borne pathogen Campylobacter jejuni contains multiple highly mutable sites, or contingency loci. It has been suggested that standing variation at these loci is a mechanism for rapid adaptation to a novel environment, but this phenomenon has not been shown experimentally. In previous work we showed that the virulence of C. jejuni NCTC11168 increased after serial passage through a C57BL/6 IL-10-/- mouse model of campylobacteriosis. Here we sought to determine the genetic basis of this adaptation during passage. Re-sequencing of the 1.64Mb genome to 200-500X coverage allowed us to define variation in 23 contingency loci to an unprecedented depth both before and after in vivo adaptation. Mutations in the mouse-adapted C. jejuni were largely restricted to the homopolymeric tracts of thirteen contingency loci. These changes cause significant alterations in open reading frames of genes in surface structure biosynthesis loci and in genes with only putative functions. Several loci with open reading frame changes also had altered transcript abundance. The increase in specific phases of contingency loci during in vivo passage of C. jejuni, coupled with the observed virulence increase and the lack of other types of genetic changes, is the first experimental evidence that these variable regions play a significant role in C. jejuni adaptation and virulence in a novel host.
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影响因子：
2
作者：
Chatre, Pierre;Haenni, Marisa;Madec, Jean-Yves
通讯作者：
Madec, Jean-Yves
DOI：
10.1073/pnas.111165798
发表时间：
2001-06-05
影响因子：
11.1
作者：
Brown, EG;Liu, H;Nesrallah, M
通讯作者：
Nesrallah, M
影响因子：
2.2
作者：
Acke, E.;McGill, K.;Whyte, P.
通讯作者：
Whyte, P.
影响因子：
2.8
作者：
Hitchen, Paul;Brzostek, Joanna;Linton, Dennis
通讯作者：
Linton, Dennis
影响因子：
3.6
作者：
Guerry, P;Ewing, CP;Logan, S
通讯作者：
Logan, S