Expansion of the antimicrobial peptide repertoire in the invasive ladybird Harmonia axyridis.
Expansion of the antimicrobial peptide repertoire in the invasive ladybird Harmonia axyridis.
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DOI：
10.1098/rspb.2012.2113
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发表时间：
2013-01-07
期刊：
影响因子：
--
通讯作者：
Vogel H
中科院分区：
文献类型：
--
作者：
Vilcinskas A;Mukherjee K;Vogel H
异色瓢虫（Harmonia axyridis）是入侵生物学中的模式物种，因为它对病原体有很强的抵抗力，并且具有超越本地瓢虫的能力。该物种的入侵成功可能反映了其适应良好的免疫系统，我们通过分析转录组和表征未经处理的甲虫以及受到细菌和真菌挑战的甲虫的免疫基因库来验证这一假设。我们发现，大多数H。异色瓢虫免疫相关基因的多样性与参比甲虫赤拟谷盗中的对应基因相似，但编码抗菌肽和抗菌蛋白（AMP）的基因出现了前所未有的扩增。我们确定了超过50个假定的AMP属于7个不同的基因家族，和许多相应的基因被显示通过定量实时RT-PCR诱导的免疫刺激的甲虫。抗菌肽的诱导率最高的挑战甲虫表现出广泛和有效的活性，对革兰氏阴性细菌和昆虫病原真菌。H.因此，异色菌至少部分归因于其免疫系统的更高效率，特别是AMP基因家族的扩增及其对病原体的应答诱导。
The harlequin ladybird beetle Harmonia axyridis has emerged as a model species in invasion biology because of its strong resistance against pathogens and remarkable capacity to outcompete native ladybirds. The invasive success of the species may reflect its well-adapted immune system, a hypothesis we tested by analysing the transcriptome and characterizing the immune gene repertoire of untreated beetles and those challenged with bacteria and fungi. We found that most H. axyridis immunity-related genes were similar in diversity to their counterparts in the reference beetle Tribolium castaneum, but there was an unprecedented expansion among genes encoding antimicrobial peptides and proteins (AMPs). We identified more than 50 putative AMPs belonging to seven different gene families, and many of the corresponding genes were shown by quantitative real-time RT–PCR to be induced in the immune-stimulated beetles. AMPs with the highest induction ratio in the challenged beetles were shown to demonstrate broad and potent activity against Gram-negative bacteria and entomopathogenic fungi. The invasive success of H. axyridis can therefore be attributed at least in part to the greater efficiency of its immune system, particularly the expansion of AMP gene families and their induction in response to pathogens.
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影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
--
作者：
Fang, Weiguo;Bidochka, Michael J.
通讯作者：
Bidochka, Michael J.
影响因子：
3.9
作者：
Shatters, Robert G., Jr.;Boykin, Laura M.;Weathersbee, A. A., III
通讯作者：
Weathersbee, A. A., III
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD
影响因子：
9.2
作者：
Facon, Benoit;Hufbauer, Ruth A.;Estoup, Arnaud
通讯作者：
Estoup, Arnaud