Creating perfused functional vascular channels using 3D bio-printing technology.
Creating perfused functional vascular channels using 3D bio-printing technology.
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DOI：
10.1016/j.biomaterials.2014.05.083
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发表时间：
2014-09
期刊：
影响因子：
14
通讯作者：
Dai, Guohao
中科院分区：
文献类型：
--
作者：
Lee, Vivian K.;Kim, Diana Y.;Ngo, Haygan;Lee, Young;Seo, Lan;Yoo, Seung-Schik;Vincent, Peter A.;Dai, Guohao
我们开发了一种使用3D生物打印技术的方法，仅使用细胞和生物基质创建具有灌注开放腔的功能性体外血管通道。所制造的血管具有紧密、汇合的内皮衬里，对血浆蛋白和高分子量葡聚糖分子都具有屏障功能。在生理流动条件下，流体血管通道能够以500万个细胞/mL的密度支持距离达5 mm的组织的活力。在静态培养的血管通道中，观察到从血管表面活跃的血管生成萌芽，而生理流动强烈抑制了这一过程。本研究报告了基因表达分析，以显示该血管模型在血管生物学研究中的潜力。该方法在使用3D生物打印技术的血管化组织制造中具有巨大的潜力，因为血管通道是同时创建的，同时细胞和基质以所需的3D图案打印在通道周围。它也可以作为一个独特的实验工具，研究血管重塑与细胞外基质和成熟过程中的三维流动条件下的基本机制。
We developed a methodology using 3D bio-printing technology to create a functional in vitro vascular channel with perfused open lumen using only cells and biological matrices. The fabricated vasculature has a tight, confluent endothelium lining, presenting barrier function for both plasma protein and high-molecular weight dextran molecule. The fluidic vascular channel is capable of supporting the viability of tissue up to 5mm in distance at 5 million cells/mL density under the physiological flow condition. In static-cultured vascular channels, active angiogenic sprouting from the vessel surface was observed whereas physiological flow strongly suppressed this process. Gene expression analysis were reported in this study to show the potential of this vessel model in vascular biology research. The methods have great potential in vascularized tissue fabrication using 3D bio-printing technology as the vascular channel is simultaneously created while cells and matrix are printed around the channel in desired 3D patterns. It can also serve as a unique experimental tool for investigating fundamental mechanisms of vascular remodeling with extracellular matrix and maturation process under 3D flow condition.
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DOI：
10.1073/pnas.0400164101
发表时间：
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影响因子：
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