Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression
Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression
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绵羊黄体中二氢睾酮的合成及其黄体退化的潜在机制
DOI：
10.1002/jcp.28159
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发表时间：
2019-01
影响因子：
5.6
通讯作者：
华永丽
中科院分区：
文献类型：
--
作者：
肖龙菲;宋亮丽;胡俊杰;张全伟;段宏伟;张勇;赵兴绪;华永丽
黄体退化是一个复杂的生理过程。已有研究表明，双氢睾酮（DHT）可能参与调节CL消退，但其机制尚不清楚。在这项研究中，我们评估了两种亚型的DHT合成酶5α-还原酶（5α-red 1和5α-red 2）和雄激素受体（AR）在绵羊CL中的定位，并通过免疫组织化学、定量真实的-时间聚合酶链反应和蛋白质印迹分析研究了5α-red 1、5α-red 2、AR和DHT在CL不同黄体期（早期、中期和晚期）的水平。此外，我们培养了中期CL的黄体细胞，并用不同浓度的DHT（10 - 10-10 - 6 M）和AR拮抗剂氟替卡松（10 - 5 M）处理它们，以评估DHT是否参与调节孕酮（P4）分泌和孕酮核受体（PGR）表达，以及这些作用是否受AR通路调节。同时观察了DHT和Fluoride对前列腺素F2α（PGF 2 α）分泌及凋亡基因和蛋白表达的影响。我们的结果表明，5α-red 1，5α-red 2和AR在CL中表达，并且它们的表达和DHT水平在黄体期发生变化。DHT介导P4和PGF 2 α的分泌、PGR和凋亡基因及蛋白的表达。DHT对CL的影响至少部分受AR通路的调节。本研究揭示了双氢睾酮对绵羊CL消退的作用机制，为进一步探讨雄激素对CL功能的调节奠定了基础。
Corpus luteum (CL) regression is a complex physiological process. Previous studies have shown that dihydrotestosterone (DHT) may be involved in regulating CL regression, but the mechanism is still unclear. In this study, we evaluated the localization of the two isoforms of DHT synthetase 5α‐reductase (5α‐red1 and 5α‐red2) and androgen receptor (AR) in sheep CL, and investigated 5α‐red1, 5α‐red2, AR, and DHT levels at different luteal stages of CL (early, middle, and late phase) by immunohistochemistry, quantitative real‐time polymerase chain reaction, and western blot analysis. Moreover, we cultured luteal cells from middle phase CL and treated them with different concentrations of DHT (10−10–10 −6 M) and the AR antagonist flutamide (10 −5 M), to evaluate whether DHT is involved in the regulation of progesterone (P4) secretion and progesterone nuclear receptor (PGR) expression and whether these effects are regulated by the AR pathway. We also investigated the effects of DHT and flutamide on prostaglandin F2α (PGF2α) secretion and apoptotic gene and protein expression. Our results showed that 5α‐red1, 5α‐red2, and AR were expressed in the CL, and their expression and DHT levels were changed during the luteal phase. DHT was involved in mediating P4 and PGF2α secretion and PGR and apoptotic gene and protein expression. The effects of DHT on CL were at least partially regulated by the AR pathway. This study reveals the mechanism of action of DHT on sheep CL regression and lays the foundation for further exploration of androgen regulation of CL function.
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DOI：
10.1038/jcbfm.2008.115
发表时间：
2009-02
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/s0140-6736(18)31578-2
发表时间：
1890-04
期刊：
The Hospital
影响因子：
--
作者：
Samir Guglani
通讯作者：
Samir Guglani
影响因子：
2.7
作者：
CONWAY, BA;MILLS, TM
通讯作者：
MILLS, TM
影响因子：
3.6
作者：
Bo R. Rueda;K. I. Tilly;I. Botros;Peter D. Jolly;Thomas R. Hansen;Patricia B. Hoyer;Jonathan L. Tilly-Jonathan
通讯作者：
Bo R. Rueda;K. I. Tilly;I. Botros;Peter D. Jolly;Thomas R. Hansen;Patricia B. Hoyer;Jonathan L. Tilly-Jonathan
影响因子：
4
作者：
Misao, R;Nakanishi, Y;Tamaya, T
通讯作者：
Tamaya, T