Evaluation of a transtympanic delivery system in Mus musculus for extended release steroids.
Evaluation of a transtympanic delivery system in Mus musculus for extended release steroids.
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DOI：
10.1016/j.ejps.2018.01.020
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Berkland CJ
中科院分区：
文献类型：
--
作者：
Dormer NH;Nelson-Brantley J;Staecker H;Berkland CJ
本研究评价了一种用于治疗内耳疾病的新型缓释给药系统。该平台技术由成膜剂（FFA）和微球组分组成，以定位和延长耳内的药物递送。研究评价了具有多个囊封的微球的溶出动力学，测试了各种FFA，以及定位于小鼠体内圆窗膜的能力。在Orbis Biosciences和堪萨斯大学医学中心完成了研究。结合体外表征，评价了含红外染料的微球制剂在C57/BL 6小家鼠中35天的圆窗膜（RWM）定位和一般耐受性。使用醋酸纤维素膜上的直立扩散池进行体外表征，通过高效液相色谱法定量药物含量。在麻醉下用27 GA针和2.0 μ L注射体积对小家鼠进行含红外染料微球的给药。体外溶解证明了FFA与微球平台释放类固醇、蛋白质、肽和核酸至少一个月的能力，而尸检显示FFA与染料负载的微球保持定位于小家鼠RWM相同时间段的能力，具有良好的耐受性。组合FFA和微球用于局部药物递送可以实现成本有效的、延长释放的新的和现有的小分子、蛋白质、肽和核酸局部递送至内耳。
The current investigation evaluated a novel extended release delivery system for treating inner ear diseases. The platform technology consists of a film forming agent (FFA) and microsphere component to localize and extend drug delivery within the ear. Studies evaluated dissolution kinetics of microspheres with multiple encapsulates, testing of a variety of FFAs, and ability to localize to the round window membrane in mice in vivo. Studies were completed at Orbis Biosciences and The University of Kansas Medical Center In conjunction with in vitro characterization, an infrared dye-containing microsphere formulation was evaluated for round window membrane (RWM) localization and general tolerability in C57/BL6 Mus musculus for 35 days. In vitro characterization was performed using upright diffusion cells on cellulose acetate membranes, with drug content quantified by high performance liquid chromatography. Mus musculus dosing of infrared dye-containing microspheres was performed under anesthesia with a 27 GA needle and 2.0 uL injection volume. In vitro dissolution demonstrates the ability of the FFA with microsphere platform to release steroids, proteins, peptides, and nucleic acids for at least one month, while necroscopy shows the ability of the FFA with dye-loaded microspheres to remain localized to Mus musculus RWM for the same period of time, with favorable tolerability. Combining FFA and microsphere for localized drug delivery may enable cost-effective, extended release local delivery to the inner ear of new and existing small molecules, proteins, peptides, and nucleic acids.
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影响因子：
16.1
作者：
Swan, Erin E. Leary;Mescher, Mark J.;Sewell, William F.;Tao, Sarah L.;Borenstein, Jeffrey I.
通讯作者：
Borenstein, Jeffrey I.
影响因子：
4.9
作者：
Berkland, C;Cox, A;Pack, DW
通讯作者：
Pack, DW
影响因子：
8.2
作者：
Zhou, Xiang-lian;He, Jin-tian;Jiang, Yang
通讯作者：
Jiang, Yang
影响因子：
3.9
作者：
Chaw, CS;Yang, YY;Phan, TT
通讯作者：
Phan, TT
DOI：
10.1007/s10162-007-0084-9
发表时间：
2007-09-01
影响因子：
2.4
作者：
So, Hongseob;Kim, Hyungjin;Park, Raekil
通讯作者：
Park, Raekil