TISdb: a database for alternative translation initiation in mammalian cells.
TISdb: a database for alternative translation initiation in mammalian cells.
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DOI：
10.1093/nar/gkt1085
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发表时间：
2014-01
影响因子：
14.9
通讯作者：
Qian SB
中科院分区：
文献类型：
--
作者：
Wan J;Qian SB
正确选择mrna上的翻译起始位点（TIS）对于产生所需的蛋白质产物至关重要。最近的研究使用核糖体分析技术发现了令人惊讶的各种潜在的TIS位点，除了带注释的开始密码子。流行的替代翻译在多样性和复杂性方面重塑了蛋白质组的景观。为了确定哺乳动物细胞中转录组的隐藏编码潜力，我们开发了全球翻译起始测序（GTI-Seq），以接近单个核苷酸的分辨率绘制全基因组TIS位置。为了促进替代翻译的研究，我们基于多次GTI-Seq复制，创建了一个从人和小鼠细胞系中鉴定出的替代TIS位点的数据库。可在http://tisdb.human.cornell.edu上获得的TISdb包括4961个人类基因的6991个TIS位点和5668个小鼠基因的9973个TIS位点。资讯科技资料库网站提供简单的浏览器介面，供查询高可信度资讯科技资料库网站及其相关的开放阅读框架。搜索结果的输出在转录异构体的背景下提供了对TIS信息的用户友好的可视化。总之，数据库中的信息为哺乳动物细胞的替代翻译提供了一个简单的参考，并将支持未来研究新的翻译产品。
Proper selection of the translation initiation site (TIS) on mRNAs is crucial for the production of desired protein products. Recent studies using ribosome profiling technology uncovered a surprising variety of potential TIS sites in addition to the annotated start codon. The prevailing alternative translation reshapes the landscape of the proteome in terms of diversity and complexity. To identify the hidden coding potential of the transcriptome in mammalian cells, we developed global translation initiation sequencing (GTI-Seq) that maps genome-wide TIS positions at nearly a single nucleotide resolution. To facilitate studies of alternative translation, we created a database of alternative TIS sites identified from human and mouse cell lines based on multiple GTI-Seq replicates. The TISdb, available at http://tisdb.human.cornell.edu, includes 6991 TIS sites from 4961 human genes and 9973 TIS sites from 5668 mouse genes. The TISdb website provides a simple browser interface for query of high-confidence TIS sites and their associated open reading frames. The output of search results provides a user-friendly visualization of TIS information in the context of transcript isoforms. Together, the information in the database provides an easy reference for alternative translation in mammalian cells and will support future investigation of novel translational products.
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影响因子：
9.8
作者：
Schwab SR;Shugart JA;Horng T;Malarkannan S;Shastri N
通讯作者：
Shastri N
影响因子：
16
作者：
Liu, Botao;Han, Yan;Qian, Shu-Bing
通讯作者：
Qian, Shu-Bing
影响因子：
56.9
作者：
Starck, Shelley R.;Jiang, Vivian;Shastri, Nilabh
通讯作者：
Shastri, Nilabh
DOI：
10.1073/pnas.1207846109
发表时间：
2012-09-11
影响因子：
11.1
作者：
Lee, Sooncheol;Liu, Botao;Qian, Shu-Bing
通讯作者：
Qian, Shu-Bing
影响因子：
64.5
作者：
Sonenberg N;Hinnebusch AG
通讯作者：
Hinnebusch AG